Search bioRxiv⌕ Search

Biology subjects

Mall, G.

Publications and source records attributed to Mall, G..

3 recordsLinked to original sources

Reconstructing Ambient Temperature in Forensic Death Time Estimation

Ambient temperature TA has a strong impact on temperature based time since death estimation (TTDE). Frequently TA is lowered instantaneously at a time t0 from a start value TA0 to TA1 < TA0 e.g. by window or door opening. We focus on reconstructing TA0 and t0. TTDE literature suggests temperature measurements in closed compartments as e.g. cupboards or neighboring rooms, where TA0 could have been preserved after t0. We aim to estimate t0 and TA0 from temperature measurements TZ(t) in closed compartments Z at times t > t0. We obtained promising results assuming Newtonian cooling for boxes filled with air, with heaps of clothes or even with books in two different experimental scenarios. Two different parameter estimators, (t0^, TA0^) forsingle quadruple temperature measurement and (t0*, TA0*) for N quadruple measurements were tested. Our results in a climate chamber were partially appropriate for TTDE input. A decline at time t0 from TA0 = 22.5{degrees}C {downarrow} TA1 = 14{degrees}C was reconstructed at t = t0 + 95min with relative deviations {rho}t0^ = 27% and {rho}TA0^ = 19% relative to t - t0 and TA0 - TA1 respectively, for N = 1 quadruple measurement with span {Delta}t = 50min. For N = 200 quadruple measurements in [t = t0 + 95min, t = t0 + 295min] we found {rho}t0^ = 5% and {rho}TA0^ = 11% with the same {Delta}t. Further research is necessary to guarantee applicability in routine casework. We will investigate more elaborate cooling models, estimation algorithms, and evaluation localization.

biophysics↗

Expression of human CEACAMs promotes inflammation and organ damage during systemic Candida albicans infection in mice

Invasive candidiasis is a fungal infection characterized by a high mortality rate. CEACAM family receptors play a crucial role in regulating innate responses of both leukocytes and epithelia. Human CEACAM3, CEACAM5 and CEACAM6 receptors recognize C. albicans and are expressed in transgenic CEABAC10 mice. In a murine C. albicans infection model, CEABAC10 mice exhibited a shortened survival period attributed to an early cytokine storm, an exacerbated acute phase response, and heightened systemic inflammation compared to their wild-type littermates. The livers and kidneys of CEABAC10 mice displayed intensified purulent necrotizing inflammation, accompanied by increased infiltration of neutrophils and macrophages. Our in vivo and in vitro data indicated that the expression of CEACAM6 on monocytes of CEABAC10 mice caused the elevated cytokine levels and the subsequent exacerbation of the acute phase response upon C. albicans infection, resulting in decreased survival.

immunology↗

Early postmortem mapping of SARS-CoV-2 RNA in patients with COVID-19 and correlation to tissue damage

Clinical observations indicate that COVID-19 is a systemic disease. An investigation of the viral distribution within the human body in correlation to tissue damage can help understanding the pathophysiology of SARS-CoV-2 infection.We present a detailed mapping of viral RNA in 61 tissues and organs of 11 deceased patients with the diagnosis COVID-19. The autopsies were performed within the (very) early postmortem interval (mean: 5.6 hours) to avoid bias due to viral RNA and tissue degradation. Viral loads, blood levels of cytokines, prothrombotic factors as well as macro- and micro-morphology were correlated.Very high (> 104 copies/ml) viral loads were detected in the lungs of most patients and then correlated to severe tissue damage. Intact viral particles could be verified in the lung tissue by transmission electron microscopy. Viral loads in the lymph nodes were associated with a loss of follicular architecture. Viral RNA was detected throughout further extra-pulmonary tissues and organs without visible tissue damage. Inflammatory cytokines as well as the prothrombotic factors were elevated in all patients.In conclusion, the dissemination of SARS-CoV-2-RNA throughout the body supports the hypothesis of a maladaptive host response with viremia and multi-organ dysfunction.View Full Text

immunology↗