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bioRxiv · 10.1101/2020.06.15.151746

Exposure to artemisinin at the trophozoite stage increases sexual conversion rates in the malaria parasite Plasmodium falciparum

Portugaliza, H. P.·Miyazaki, S.·Geurten, F. J. A.·Pell, C.·Rosanas-Urgell, A.·Janse, C. J.·Cortes, A.
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Abstract

Malaria transmission is dependent on formation of gametocytes in the human blood. The sexual conversion rate, the proportion of asexual parasites that convert into gametocytes at each multiplication cycle, is variable and reflects the relative parasite investment between transmission and maintaining the infection. The impact of environmental factors such as drugs on sexual conversion rates is not well understood. We developed a robust assay using gametocyte-reporter parasite lines to accurately measure the impact of drugs on conversion rates, independently from their gametocytocidal activity. We found that exposure to subcurative doses of the frontline antimalarial drug dihydroartemisinin (DHA) at the trophozoite stage resulted in a ~4-fold increase in sexual conversion. In contrast, no increase was observed when ring stages were exposed or in cultures in which sexual conversion was stimulated by choline depletion. Our results reveal a complex relationship between antimalarial drugs and sexual conversion, with potential public health implications.

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Portugaliza, H. P., Miyazaki, S., Geurten, F. J. A., Pell, C., Rosanas-Urgell, A., Janse, C. J., Cortes, A.. 2020-06-15. Exposure to artemisinin at the trophozoite stage increases sexual conversion rates in the malaria parasite Plasmodium falciparum. https://doi.org/10.1101/2020.06.15.151746

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