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bioRxiv · 10.1101/2020.05.21.109546

A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation

Muruato, A. E.·Fontes-Garfias, C. R.·Ren, P.·Garcia-Blanco, M. A.·Menachery, V. D.·Xie, X. D.·Shi, P.-Y.
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Abstract

Virus neutralization remains the gold standard for determining antibody efficacy. Therefore, a high-throughput assay to measure SARS-CoV-2 neutralizing antibodies is urgently needed for COVID-19 serodiagnosis, convalescent plasma therapy, and vaccine development. Here we report on a fluorescence-based SARS-CoV-2 neutralization assay that detects SARS-CoV-2 neutralizing antibodies in COVID-19 patient specimens and yields comparable results to plaque reduction neutralizing assay, the gold standard of serological testing. Our approach offers a rapid platform that can be scaled to screen people for antibody protection from COVID-19, a key parameter necessary to safely reopen local communities.

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Muruato, A. E., Fontes-Garfias, C. R., Ren, P., Garcia-Blanco, M. A., Menachery, V. D., Xie, X. D., Shi, P.-Y.. 2020-05-22. A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation. https://doi.org/10.1101/2020.05.21.109546

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