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bioRxiv · 10.1101/2020.05.01.073387

PSGL-1 blocks SARS-CoV-2 S protein-mediated virus attachment and infection of target cells

He, S.·Hetrick, B.·Dabbagh, D.·Akhrymuk, I. V.·Kehn-Hall, K.·Freed, E. O.·Wu, Y.
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Abstract

P-selectin glycoprotein ligand-1 (PSGL-1) is a cell surface glycoprotein that binds to P-, E-, and L-selectins to mediate the tethering and rolling of immune cells on the surface of the endothelium for cell migration into inflamed tissues. PSGL-1 has been identified as an interferon-γ (INF-γ)-regulated factor that restricts HIV-1 infectivity, and has recently been found to possess broad-spectrum antiviral activities. Here we report that the expression of PSGL-1 in virus-producing cells impairs the incorporation of SARS-CoV and SARS-CoV-2 spike (S) glycoproteins into pseudovirions and blocks virus attachment and infection of target cells. These findings suggest that PSGL-1 may potentially inhibit coronavirus replication in PSGL-1+ cells.Competing Interest StatementThe authors have declared no competing interest.View Full Text

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He, S., Hetrick, B., Dabbagh, D., Akhrymuk, I. V., Kehn-Hall, K., Freed, E. O., Wu, Y.. 2020-05-02. PSGL-1 blocks SARS-CoV-2 S protein-mediated virus attachment and infection of target cells. https://doi.org/10.1101/2020.05.01.073387

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