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bioRxiv · 10.1101/2020.03.27.006247

Structure based virtual screening identifies novel competitive inhibitors for the sialoglycan binding protein Hsa

Abstract

Infective endocarditis (IE) is a cardiovascular disease often caused by bacteria of the viridans group of streptococci, which includes Streptococcus gordonii and Streptococcus sanguinis. Previous research has found that a serine-rich repeat (SRR) proteins on the S. gordonii bacterial surface play a critical role in pathogenesis by facilitating bacterial attachment to sialyated glycans displayed on human platelets. Despite its important role in disease progression, there are currently no anti-adhesive drugs available on the market. Here, we performed structure-based virtual screening using an ensemble docking approach followed by consensus scoring to identify novel inhibitors against the sialoglycan binding domain of the SRR adhesin protein Hsa from the S. gordonii strain DL1. In silico cross screening against the glycan binding domains of closely related SRR proteins from five other S. gordonii or S. sanguinis strains was also performed to further reduce false positives. Using our in silico screening strategy we successfully predicted nine compounds which were able to displace the native ligand (sialyl-T antigen) in an in vitro assay and bind competitively to adhesin protein Hsa ([~]20% hit rate).

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BibTeXRIS

Agarwal, R., Bensing, B. A., Mi, D., Vinson, P. N., Baudry, J., Iverson, T. M., Smith, J. C.. 2020-03-29. Structure based virtual screening identifies novel competitive inhibitors for the sialoglycan binding protein Hsa. https://doi.org/10.1101/2020.03.27.006247

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