Altered structure and stability of bat-prey interaction networks in logged tropical forests revealed by metabarcoding

Abstract

O_LIHabitat degradation is pervasive across the tropics and is particularly acute in Southeast Asia, with major implications for biodiversity. Much research has addressed the impact of degradation on species diversity; however, little is known about how ecological interactions are altered, including those that constitute important ecosystem functions such as pest consumption. C_LIO_LIWe examined how rainforest degradation alters trophic interaction networks linking insectivorous bats and their prey. We used DNA metabarcoding to study the diets of forest-dwelling insectivorous bat species, and compared bat-prey interaction networks between old growth forest and forest degraded by logging in Sabah, Borneo. C_LIO_LIWe predicted that rainforest degradation would cause measurable reductions in the numbers of prey consumed by individual bats, and that this degradation would yield networks in logged forest with lower functional complementarity, modularity and nestedness than those in old growth forest. C_LIO_LICompared to bats in old growth rainforest, bats in logged sites consumed a lower diversity of prey. Their interaction networks were less nested and had a more modular structure in which bat species had lower closeness centrality scores than in old growth forest. These network structures were associated with reduced network redundancy and thus increased vulnerability to perturbations in logged forests. C_LIO_LIOur results show how ecological interactions change between old growth and logged forests, with potentially negative implications for ecosystem function and network stability. We also highlight the potential importance of insectivorous bats in consuming invertebrate pests. C_LI Malay abstractO_LIDegradasi habitat merupakan suatu fenomena yang berleluasa dikawasan tropika, terutamanya di Asia Tenggara dengan implikasi yang besar ke atas biodiversiti. Banyak kajian telahpun meneliti impak degradasi habitat atas kepelbagaian spesis. Walau bagaimanapun, dari segi mana interaksi ekologi diubah suai kurang diselidik, termasuk interaksi yang membentuk fungsi ekosistem yang penting seperti pemakanan binatang perosak. C_LIO_LIKami telah memeriksa bagaimana degradasi hutan hujan tropika dapat mengubah suai interaksi antara tahap trofik yang menghubungkan kelawar yang memakan serangga dan mangsa mereka. Kami telah menggunakan "DNA metabarcoding" untuk mengenal pasti kandungan artropod dalam sampel najis kelawar and membandingkan jaringan interaksi kelawar dan mangsa mereka diantara hutan dara dan hutan yang telah dibalak di Sabah, Borneo. C_LIO_LIKami meramalkan bahawa degradasi hutan hujan akan menyebabkan kekurangan dalam bilangan nod mangsa yang dimakan oleh setiap individu kelawar yang dapat diukur. Degradasi ini pula boleh menghasilkan jaringan yang mempunyai fungsi saling melengkapi dan modulariti yang rendah, dan lebih berkelompok atau "mempunyai "nestedness" yang lebih tinggi di hutan yang dibalak berbanding hutan dara. C_LIO_LIKelawar di kawasan hutan yang dibalak memakan diversiti mangsa yang lebih rendah dengan kelawar di habitat hutan hujan dara. Jaringan-jaringan interaksi mereka kurang berkelompok dan mempunyai stuktur yang lebih modular dimana spesis kelawar mempunyai pemarkahan kerapatan berpusat yang lebih rendah daripada sepesis kelawar di hutan dara. Struktur-struktur jaringan ini berkait dengan lebihan jaringan atau "network redundancy" yang lebih rendah and ini membawa kepada kerentantan yang meningkat terhadap gangguan luar di hutan yang telah dibalak. C_LIO_LIKeputusan kami menunjukkan bagaimana interaksi ekologi berubah diantara hutan dara dan hutan yang dibalak, dengan potensi implikasi negatif untuk fungsi ekosistem dan kestabilan jaringan. Kami juga telah menunjukkan potensi kepentingan kelawar yang memakan serangga dalam fungsi mereka untuk makan perosak invertebrat. C_LI Data Accessibility StatementData are currently archived at the Centre for Ecology and Hydrology Environmental Information Data Centre (https://doi.org/10.5285/8b106445-d8e0-482c-b517-5a372a09dc91) and will be released from embargo following publication. Specific analysis scripts are available on GitHub with links given in the manuscript and will be archived on Zenodo prior to publication. Statement of authorshipSR, EC, DHB, MS and OTL conceived the project, DHB, VK and JB undertook field collections and laboratory work, DHB analysed the data with input from EC, and DHB wrote the manuscript with input from all authors.