bioRxiv · 10.1101/2020.01.28.911115
A cell atlas of human thymic development defines T cell repertoire formation
Abstract
The thymus provides a nurturing environment for the differentiation and selection of T cells, a process orchestrated by their interaction with multiple thymic cell types. We utilised single-cell RNA-sequencing (scRNA-seq) to create a cell census of the human thymus and to reconstruct T-cell differentiation trajectories and T-cell receptor (TCR) recombination kinetics. Using this approach, we identified and located in situ novel CD8+ T-cell populations, thymic fibroblast subtypes and activated dendritic cell (aDC) states. In addition, we reveal a bias in TCR recombination and selection, which is attributed to genomic position and suggests later commitment of the CD8+ T-cell lineage. Taken together, our data provide a comprehensive atlas of the human thymus across the lifespan with new insights into human T-cell development.
Source connections
Explore related subjects
Keep this discovery
Explore connections, maps & timelines
Park, J.-E., Botting, R. A., Dominguez Conde, C., Popescu, D.-M., Lavaert, M., Kunz, D. J., Stephenson, E., Ragazzini, R., Tuck, E., Wilbrey-Clark, A. L., Ferdinand, J. R., Webb, S., Maunder, D., Vandamme, N., Mahbubani, K. T., Polanski, K., Mamanova, L., Fuller, A., Filby, A., Reynolds, G., Dixon, D., Saeb-Parsy, K., Lisgo, S., Henderson, D., Vento-Tormo, R., Meyer, K. B., Saeys, Y., Bonfanti, P., Behjati, S., Clatworthy, M. R., Taghon, T., Haniffa, M., Teichmann, S. A.. 2020-01-28. A cell atlas of human thymic development defines T cell repertoire formation. https://doi.org/10.1101/2020.01.28.911115
Cite the original work for its findings. Save a collection to share your selection of sources.