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bioRxiv · 10.1101/144535

Protein Profiling In Cancer Cell Lines And Tumor Tissue Using Reverse Phase Protein Arrays

Jing, X.·Wang, W.·Gauthier, N. P.·Kaushik, P.·Root, A.·Stein, R. R.·Korkut, A.·Sander, C.
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Abstract

Reverse phase protein array (RPPA) technology is an antibody-based high-throughput assay for protein profiling of biological specimens that allows for many measurements with very small amounts of cell lysate. Here, we report the sensitivity, reproducibility, and accuracy of a particular RPPA platform called Zeptosens. We customized the RPPA protocol for our in-house setup, and measured more than 80 total protein and phospho-protein levels in various cancer samples, including cell lines, organoids, tumor chunks, core needle biopsies, and laser-capture microdissected tissue samples. We discuss pros and cons of the RPPA platform, and describe results from profiling 15 cancer cell line cells using RPPA.

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Jing, X., Wang, W., Gauthier, N. P., Kaushik, P., Root, A., Stein, R. R., Korkut, A., Sander, C.. 2017-05-31. Protein Profiling In Cancer Cell Lines And Tumor Tissue Using Reverse Phase Protein Arrays. https://doi.org/10.1101/144535

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