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bioRxiv · 10.1101/100214

An Improved Crystal Violet Assay for Biofilm Quantification in 96-Well Microtitre Plate

Shukla, S. K.·Rao, T. S.
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Abstract

Microplates are essential tools for biofilm research since it allows high throughput screening of biofilm forming strains or in the assay of anti-biofilm drugs. However, 96 well microtitre plate based assays share the issue of \"edge effect\". The primary cause of the \"edge effect\" phenomenon is evaporation. As edge effect causes a significant increase in plate rejection rate by introducing experimental error, we improvised the classical crystal violet assay to reduce water loss from the peripheral wells. The improvised method showed a significant reduction in edge effect and minimised error in crystal violet assay

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Shukla, S. K., Rao, T. S.. 2017-01-13. An Improved Crystal Violet Assay for Biofilm Quantification in 96-Well Microtitre Plate. https://doi.org/10.1101/100214

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