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bioRxiv · 10.1101/048603

Third-generation sequencing and the future of genomics

Hayan Lee·James Gurtowski·Shinjae Yoo·Maria Nattestad·Shoshana Marcus·Sara Goodwin·W. Richard McCombie·Michael Schatz
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Abstract

Third-generation long-range DNA sequencing and mapping technologies are creating a renaissance in high-quality genome sequencing. Unlike second-generation sequencing, which produces short reads a few hundred base-pairs long, third-generation single-molecule technologies generate over 10,000 bp reads or map over 100,000 bp molecules. We analyze how increased read lengths can be used to address longstanding problems in de novo genome assembly, structural variation analysis and haplotype phasing.

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Hayan Lee, James Gurtowski, Shinjae Yoo, Maria Nattestad, Shoshana Marcus, Sara Goodwin, W. Richard McCombie, Michael Schatz. 2016-04-13. Third-generation sequencing and the future of genomics. https://doi.org/10.1101/048603

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