Ag+-regulated aptamers to thrombin
Nucleic acid aptamers are artificial recognition elements with high potential in biotechnology. Approaches for aptamer affinity optimization and activity regulation are required for aptamer-based nanodevices. Here thrombin aptamer affinity was increased by a single nucleotide modification with 7,8-dihydro-8-oxo-1,N6-ethenoadenine. Whereas a double modification makes the aptamer activity Ag+-dependent.
biochemistry↗