Biology subjects

Joyce, A. S.

Publications and source records attributed to Joyce, A. S..

3 recordsLinked to original sources

bioRxiv2026

Evidence from three taxonomically distinct species for a non-AhR mechanism of developmental neurotoxicity of an environmentally derived mixture of polycyclic aromatic hydrocarbons

Typical environmental exposures to the toxic class of chemicals known as polycyclic aromatic hydrocarbons (PAHs) involve complex mixtures; however, relatively few mechanistic toxicity studies have evaluated them as environmental mixtures, instead focusing on individual compounds or simple mixtures. In this study, we first derived Republic Sediment Extract (REPSE), a complex PAH mixture extracted from sediment at the Republic Creosoting site of the Elizabeth River in Norfolk, Virginia. After characterizing the PAH contents of REPSE, we evaluated its mechanisms of developmental neurotoxicity in three evolutionarily distinct taxa, leveraging the unique strengths of Atlantic killifish, zebrafish, and Caenorhabditis elegans as model species, with a focus on the Aryl hydrocarbon Receptor (AhR) pathway. Embryonic REPSE exposure caused induction of CYP1A in both fish species at sub-teratogenic concentrations, consistent with activation of the canonical AhR pathway. These sub-teratogenic exposures nevertheless induced neurotoxicity across both fish species, altering neurobehavioral phenotypes in fish, and induced dopaminergic neuronal damage in worms, again at non-teratogenic concentrations. To determine whether these effects were linked to canonical AhR response pathways, we examined killifish offspring from the pollution-adapted Republic Creosoting population, which exhibited characteristic recalcitrance to CYP1A induction, but remained susceptible to the neurobehavioral effects of REPSE. The induction of neuronal damage in worms provides orthogonal evidence for a non-AhR mechanism, because C. elegans AhR is not transcriptionally activated by PAHs as in vertebrates. Further probing of potential mechanisms underlying REPSE-induced neurotoxicity in worms revealed altered neuronal redox status (roGFP) and energy availability (ATP:ADP ratio). Collectively, our multispecies approach reveals conserved mechanisms of PAH mixture neurotoxicity, including effects that extend beyond canonical AhR signaling.

pharmacology and toxicology↗

bioRxiv2026

Autoinducer-2 and acyl homoserine lactones have contrasting effects on ammonia and nitrite-oxidizing sludge

Enhancing nitrification with quorum sensing manipulation has emerged as a promising strategy to overcome rate-limiting steps. This study examined how disrupting microbial cell-to-cell communication by supplementing or quenching signal molecules regulates ammonia- and nitrite-oxidizing activity within activated sludge. Prolonged enrichment of activated sludge over 180 days yielded stable and robust nitrifying consortia, increasing the ammonia oxidation rate (AOR) from 5.4 to 9.3 mg N g-{superscript 1} VSS h-{superscript 1} and the nitrite oxidation rate (NOR) from 0.6 to 4.8 mg N g-{superscript 1} VSS h-{superscript 1}, while reducing the hydraulic residence time by 50 % (from 72 h to 36 h). Exogenous addition of oxoacyl and long-chain acyl homoserine lactone (AHL) signals further boosted AOR up to 4.5-fold higher than the control activated sludge, predominantly through transcriptional activation of the amoA gene in Nitrosomonas eutropha. Acylase-mediated AHL quenching lowered AOR to 4.8 mg N g-{superscript 1} VSS h-{superscript 1} but improved functional resilience of nitrite oxidizing bacteria by enhancing mass transfer and oxygen diffusion via smaller flocs (123 {micro}m vs 357 {micro}m in AHL-treated sludge). Conversely, elevated autoinducer-2 (AI-2) levels suppressed ammonia-oxidizing activity (AOR = 2 mg N g-{superscript 1} VSS h-{superscript 1}) yet stimulated nitrite oxidation (NOR = 36 mg N g-{superscript 1} VSS h-{superscript 1}), particularly Nitrospira, underscoring the contrasting regulatory requirements of the two nitrifying guilds. Overall, the study demonstrates that AHLs and AI-2 serve as complementary yet opposing regulators of nitrification, primarily activating ammonia oxidizers and nitrite oxidizers, respectively. Maintaining these signaling molecules within an optimal physiological window thereby offers a biologically tunable approach for synchronized ammonia and nitrite oxidation, ultimately maximizing nitrogen removal in biological treatment systems.

microbiology↗

bioRxiv2025

Assessment of developmental neurotoxicology-associated alterations in neuronal architecture and function using Caenorhabditis elegans

Few of the many chemicals that regulatory agencies are charged with assessing for risk have been carefully tested for developmental neurotoxicity (DNT). To speed up testing efforts, as well as to reduce the use of vertebrate animals, great effort is being devoted to alternate laboratory models for testing DNT. A major mechanism of DNT is altered neuronal architecture resulting from chemical exposure during neurodevelopment. Caenorhabditis elegans is a nematode that has been extensively studied by neurobiologists and developmental biologists, and to a lesser extent by neurotoxicologists. The developmental trajectory of the nervous system in C. elegans is easily visualized, normally entirely invariant, and fully mapped. Therefore, we hypothesized that C. elegans could be a powerful in vivo model to test chemicals for the potential to alter developmental patterning of neuronal architecture. To test whether this might be true, we developed a novel C. elegans DNT testing paradigm that includes exposure throughout development, examines all major neurotransmitter neuronal types for architectural alterations, and tests behaviors specific to dopaminergic, cholinergic, and glutamatergic functions. We used this paradigm to characterize the effects of early-life exposures to the developmental neurotoxicants lead, cadmium, and benzo(a)pyrene (BaP) on dopaminergic, cholinergic, and glutamatergic architecture. We also assessed whether exposures would alter neuronal specification as assessed by expression of reporter genes diagnostic of specific neurotransmitters. We identified no cases in which the apparent neurotransmitter type of the neurons we examined changed, but many in which neuronal morphology was altered. We also found that neuron-specific behaviors were altered during C. elegans mid-adulthood for populations with measured morphological neurodegeneration in earlier stages. The functional changes were consistent with the morphological changes we observed in terms of type of neuron affected. We identified changes consistent with those reported in the mammalian DNT literature, strengthening the case for C. elegans as a DNT model, and made novel observations that should be followed up in future studies.

pharmacology and toxicology↗