Site Specific Hydration Contributes to the Thermal Stability of Proteins and Protein-Complexes

Rider, R. L.; Xi, Z.; Hillaire, A.; Powell, J.; Spelbring, A.; Evans, K. A.; Lantz, C.; Scott, E.; Barondeau, D. P.; Raab, S.; Clemmer, D. E.; Laganowsky, A.; Russell, D. H.

doi:10.64898/2026.10.26.761984

bioRxiv · 10.64898/2026.10.26.761984

Site Specific Hydration Contributes to the Thermal Stability of Proteins and Protein-Complexes

Rider, R. L.·Xi, Z.·Hillaire, A.·Powell, J.·Spelbring, A.·Evans, K. A.·Lantz, C.·Scott, E.·Barondeau, D. P.·Raab, S.·Clemmer, D. E.·Laganowsky, A.·Russell, D. H.

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Abstract

Water is integral to protein structure and stability, yet ensemble measurements rarely identify the hydration interactions associated with stabilization. Here, variable-temperature electrospray ionization ion mobility mass spectrometry, conformation-resolved thermodynamic analysis, and molecular dynamics simulations are combined to examine solvent-dependent thermal stability of chymotrypsin inhibitor 2 (CI-2), a model two-state folder, and the barnase-barstar (Bn-B*) complex, which contains a highly polar binding interface. Replacing H2O with D2O raises the thermal transition temperature of both systems by approximately 3 {degrees}C, favors compact conformations, and reduces the relative thermodynamic accessibility of extended states. The conformationally resolved enthalpy-entropy compensation reveals D2O increases enthalpy for CI-2 but reduces enthalpy for Bn-B* as a result of opposing effects on the individual subunits of the complex. Molecular dynamics simulations reveal that lower solvent-accessible surface areas and fewer protein-water hydrogen bonds in D2O coexist with longer water residence times at specific structural sites. In CI-2, the strongest changes localize to the second {beta}-strand/C-terminal region; in Bn-B*, persistent hydration is concentrated at the charged protein-protein interface. Together, these results support a model in which D2O dependent stabilization is associated not only with the extent of hydration, but the site and lifetime of water interactions. More broadly, this work provides an experimental framework for resolving solvent-dependent conformational thermodynamics of protein unfolding and interactions, enabling direct comparison of free-energy landscapes across solution environments.

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Rider, R. L., Xi, Z., Hillaire, A., Powell, J., Spelbring, A., Evans, K. A., Lantz, C., Scott, E., Barondeau, D. P., Raab, S., Clemmer, D. E., Laganowsky, A., Russell, D. H.. 2026-10-28. Site Specific Hydration Contributes to the Thermal Stability of Proteins and Protein-Complexes. https://doi.org/10.64898/2026.10.26.761984

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