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bioRxiv · 10.64898/2026.10.23.761444

Hydrogel dimensionality directs mechanics-dependent nascent matrix deposition by tendon fibroblasts

Liu, J. Y.-C.·Yang, Z.·Steltzer, S. S.·Fan, M.·Navarro Salazar, E.·Liang, J.·Killian, M.·Loebel, C.
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Abstract

Tendon fibroblasts continuously synthesize and remodel extracellular matrix (ECM) in response to their surrounding mechanical environment in vivo and in vitro, yet how dimensionality regulates the deposition of newly synthesized matrix remains poorly understood. Here, we investigated nascent matrix deposition by primary mouse tail tendon fibroblasts cultured on flat two-dimensional (2D) or encapsulated within three-dimensional (3D) norbornene-modified hyaluronic acid hydrogels with tunable mechanical properties. Newly synthesized matrix was visualized using metabolic labeling with azido-galactosamine (GalNAz), which labels nascent glycoproteins deposited during culture. In 2D, increasing hydrogel elastic modulus from 10 to 25 kPa enhanced nascent matrix deposition. This response was accompanied by increased cell spreading and YAP nuclear localization, indicating increased cellular mechanosensing. In contrast, tendon fibroblasts encapsulated within enzymatically degradable 3D hydrogels showed progressive matrix deposition over time but deposited less nascent matrix in stiffer (~50 kPa) hydrogels compared with softer 5 and 10 kPa hydrogels. YAP nuclear localization was increased in softer hydrogels compared with 50 kPa hydrogels. Bulk RNA sequencing showed increased expression of tendon matrisome-associated genes in softer 3D hydrogels, including increased tenascin C and collagen type I expression. These findings demonstrate that hydrogel dimensionality determines the relationship between matrix mechanics and nascent matrix deposition. This work establishes a framework for studying how engineered microenvironments regulate the matrix-producing behavior of tendon fibroblasts and provides insight into the design of biomaterials for investigating tendon biology and matrix remodeling.

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Liu, J. Y.-C., Yang, Z., Steltzer, S. S., Fan, M., Navarro Salazar, E., Liang, J., Killian, M., Loebel, C.. 2026-10-27. Hydrogel dimensionality directs mechanics-dependent nascent matrix deposition by tendon fibroblasts. https://doi.org/10.64898/2026.10.23.761444

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