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bioRxiv · 10.64898/2026.10.16.759592

Genotype imputation improves GWAS resolution and facilitates candidate gene identification in pearl millet

Kambara, K.·Gupta, S. K.·Tsugama, D.
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Abstract

Pearl millet (Cenchrus americanus; syn. Pennisetum glaucum (L.) R. Br.) is an important cereal crop mainly grown in semi-arid areas. However, in pearl millet, genes underlying many agronomic traits remain unknown. Our previous genome-wide association study (GWAS) was based primarily on genotype data derived from restriction site-associated DNA sequencing (RAD-seq), which contained a high proportion of missing genotypes and may have limited mapping resolution. To address this limitation, we used whole-genome sequencing (WGS)-derived genotype data from 21 elite lines as a reference panel to impute missing genotypes in the RAD-seq-derived dataset. Genotype imputation reduced the proportion of missing genotypes from 82.42% to 0% and increased the mean density of non-missing genotypes from 569.61 to 1,158.09 genotypes/Mb. Leave-one-out validation yielded a mean genotype concordance of 89.9% (SD = 4.6%). GWAS using the imputed dataset showed more localized association signals for several traits. Homologs of rice SHORT INTERNODES1, Arabidopsis FLOWERING LOCUS Y, and rice EPIDERMAL PATTERNING FACTOR 2 were identified as candidates for genes regulating culm height, days to panicle emergence, and awn growth, respectively. These results demonstrate that reference-panel-based genotype imputation can increase the utility of reduced-representation genotype datasets and facilitate candidate gene identification in pearl millet.

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Kambara, K., Gupta, S. K., Tsugama, D.. 2026-10-22. Genotype imputation improves GWAS resolution and facilitates candidate gene identification in pearl millet. https://doi.org/10.64898/2026.10.16.759592

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