Single-nucleus chromatin accessibility time course of excisional wound repair in rat skin

Chai, C.; Ding, X.; Li, Y.; Liang, L.; Huang, R.; Zhang, W.; Li, R.; Xie, S.; Zeng, X.; Zhang, L.; Yuan, Y.; Wei, X.; Wang, J.; Wang, S.; Ji, S.

doi:10.64898/2026.10.15.759293

bioRxiv · 10.64898/2026.10.15.759293

Single-nucleus chromatin accessibility time course of excisional wound repair in rat skin

Chai, C.·Ding, X.·Li, Y.·Liang, L.·Huang, R.·Zhang, W.·Li, R.·Xie, S.·Zeng, X.·Zhang, L.·Yuan, Y.·Wei, X.·Wang, J.·Wang, S.·Ji, S.

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Abstract

We present a time-series single-nucleus ATAC-seq dataset from full-thickness excisional wounds in Sprague-Dawley rats. Samples were taken from unwounded skin (normal control) and at 1, 2, 5, 10, and 19 days post-wounding, spanning the inflammatory, proliferative, and remodeling phases of skin repair. We extracted nuclei with a two-step tissue dissociation protocol and prepared libraries using the DNBelab C Series Single-Nucleus ATAC Kit, then sequenced on the MGI DNBSEQ-T1 platform. After stringent quality filtering, 37,555 nuclei passed quality control; the resulting chromatin accessibility profiles cover 23 cell types: fibroblast subpopulations, keratinocytes, vascular endothelial cells, immune cells, and skin appendage cell types. This genome-wide chromatin accessibility dataset is a resource for studying regulatory dynamics during excisional wound repair and for comparing results with other wound healing systems.

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Chai, C., Ding, X., Li, Y., Liang, L., Huang, R., Zhang, W., Li, R., Xie, S., Zeng, X., Zhang, L., Yuan, Y., Wei, X., Wang, J., Wang, S., Ji, S.. 2026-10-20. Single-nucleus chromatin accessibility time course of excisional wound repair in rat skin. https://doi.org/10.64898/2026.10.15.759293

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