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bioRxiv · 10.64898/2026.10.11.758349

A miniaturized Arabidopsis thaliana - Botrytis cinerea seedling pathosystem and the GreenDayz workflow for longitudinal image-based phenotyping

Tguafaiti, I.·BRUEL, C.·CHOQUER, M.·Bournaud, C.·Poussereau, N.·Gillet, F.-X.
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Abstract

Plant-pathogen interactions are strongly influenced by host developmental stage, yet many Arabidopsis thaliana-Botrytis cinerea assays remain centred on mature rosette leaves and rely on inoculum loads that may exceed those involved in a primary infection. We established a miniaturized in vitro A. thaliana-B. cinerea seedling pathosystem initiated with a very low conidial dose (nominally ~25 conidia per plate) compatible with both destructive molecular sampling and repeated, non-destructive RGB imaging. The pathosystem was coupled to a low-cost smartphone photo station and to GreenDayZ Phenotyping, a guided image-analysis application with an intuitive graphical interface that standardizes longitudinal RGB image processing and extracts quantitative colour-based phenotypes. Together, the pathosystem and GreenDayZ Phenotyping provide an accessible and reproducible framework for longitudinal phenotyping of low-inoculum seedling infections, in which image-derived trajectories can be aligned with independent molecular readouts.

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Tguafaiti, I., BRUEL, C., CHOQUER, M., Bournaud, C., Poussereau, N., Gillet, F.-X.. 2026-10-17. A miniaturized Arabidopsis thaliana - Botrytis cinerea seedling pathosystem and the GreenDayz workflow for longitudinal image-based phenotyping. https://doi.org/10.64898/2026.10.11.758349

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