Outgrowth of Mouse Sensory Neurons is not Mediated by Regeneration Associated Genes in Early Postnatal Stages

Molina-Esteve, B.; Crugeiras, J. M.; Sanchez-Maldonado, A.; Rodriguez-Branas, M.; Ovelleiro, D.; Lago, N.; Udina, E.

doi:10.64898/2026.10.06.757081

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bioRxiv · 10.64898/2026.10.06.757081

Outgrowth of Mouse Sensory Neurons is not Mediated by Regeneration Associated Genes in Early Postnatal Stages

Molina-Esteve, B.·Crugeiras, J. M.·Sanchez-Maldonado, A.·Rodriguez-Branas, M.·Ovelleiro, D.·Lago, N.·Udina, E.

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Abstract

Peripheral neurons exhibit robust regeneration after nerve injury in adulthood; a process linked to the activation of regeneration-associated genes (RAGs). However, the mechanisms underlying nerve regeneration during early postnatal development remain poorly understood. In a previous work, we observed that motoneurons axotomized at early postnatal stages regenerate without overexpressing RAGs, contrasting with the expected RAG activation observed in postnatal day 30 (P30) mice. To determine whether this phenomenon extends to sensory neurones, we investigated two paradigmatic dorsal root ganglia populations: proprioceptive (PV) and nociceptive (TrpV1) neurones, which exhibit distinct regenerative capacities in adult mice. Following sciatic nerve crush injury at P4, P10, and P30, both neuronal subtypes demonstrated robust axonal regeneration across all postnatal stages, with nociceptors exhibiting significantly superior regeneration compared to proprioceptors. Remarkably, no substantial neuronal death was observed at any developmental timepoint. However, RNA sequencing revealed that P4 and P10 sensory neurones failed to upregulate RAGs following injury, whereas P30 neurones exhibited the expected pro-regenerative transcriptional programme. Pathway enrichment analysis confirmed that classical regeneration-associated signalling pathways were activated exclusively in young adults, whilst developmental and inflammatory pathways predominated in early postnatal stages. Using the conditioning lesion paradigm in primary dorsal root ganglia cultures, we demonstrated that only P30 neurones exhibited enhanced neurite outgrowth following conditioning lesion, whilst P4 and P10 neurones showed no conditioning effect or reduced outgrowth, respectively. These findings indicate that early postnatal sensory neurones possess intrinsic growth capacity independent of RAG activation, suggesting fundamentally distinct regenerative mechanisms across developmental stages.

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Molina-Esteve, B., Crugeiras, J. M., Sanchez-Maldonado, A., Rodriguez-Branas, M., Ovelleiro, D., Lago, N., Udina, E.. 2026-10-11. Outgrowth of Mouse Sensory Neurons is not Mediated by Regeneration Associated Genes in Early Postnatal Stages. https://doi.org/10.64898/2026.10.06.757081

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