Heat stress reveals divergent pollen viability responses across species and populations of monkeyflower

Abstract

Premise Rising temperatures can reduce plant reproductive success by disrupting pollen development, yet little is known about how reproductive heat sensitivity varies among natural populations. We evaluated pollen viability responses to elevated temperature in two closely related taxa of yellow monkeyflowers across multiple geographic scales to determine whether heat sensitivity varies among species, between populations and across elevations. Methods We conducted three common garden growth chamber experiments. First, we compared pollen viability responses to heat stress between Mimulus guttatus and members of the M. tilingii species complex. Second, we tested whether broad elevational categories explained variation in heat responses. Third, we evaluated heat sensitivity among nine eastern Sierra Nevada populations of M. guttatus spanning an elevational gradient. Pollen viability was assessed following exposure to control and heat treatments. Key results Heat exposure reduced pollen viability in all experiments. In the species comparison experiment, pollen viability decreased more strongly in the high-elevation restricted M. tilingii species complex than the widespread M. guttatus. We found substantial population-level variation in pollen viability within M. guttatus but not members of the M. tilingii complex. Broad elevational categories did not explain this variation in pollen viability responses. However, within eastern Sierra Nevada populations, reductions in pollen viability became stronger with increasing source elevation. Several populations deviated from this trend, indicating reproductive responses to heat cannot be explained by elevation alone. Conclusions Pollen heat sensitivity varies substantially among species and populations of yellow monkeyflowers. Elevation alone is insufficient to predict pollen viability responses to elevated temperatures, suggesting that local adaptation or genetic variation may contribute to reproductive heat tolerance. Some populations of M. guttatus may contain greater capacity to maintain reproductive function under future warming, highlighting the importance of considering intraspecific variation when predicting plant responses to climate change.