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bioRxiv · 10.64898/2026.09.20.752969

GPC6 Lipid Shielding Sustains WNT5A Gradients to Prevent a RASopathy-like Developmental State

Abstract

Morphogen gradients require ligands to disperse across tissues from their sites of production, yet receptor engagement can consume ligand before it reaches distant cells. This poses a particular challenge for lipid-modified WNTs, whose hydrophobic palmitoleate favours ligand retention near their source and premature receptor engagement. Here we identify a palmitoleate-binding pocket in mammalian glypican-6 (GPC6), the gene mutated in the skeletal dysplasia omodysplasia, and show that shielding of the WNT5A lipid by GPC6 is required for gradient formation. Using a separation-of-function knock-in mouse allele, we demonstrate that targeted disruption of GPC6-mediated lipid shielding collapses WNT5A gradients during skeletal development by promoting premature source-proximal signalling that depletes ligand available for distal signalling. This source-proximal WNT hyperactivation engages a previously unrecognised WNT-driven RAC-PAK-MEK-ERK cascade, generating a RASopathy-like developmental state that contributes to skeletal dysplasia. Thus, GPC6 functions as a spatial gatekeeper of WNT5A utilisation, controlling the allocation of a finite ligand pool. More broadly, these findings reveal that pathway hyperactivation can arise from spatial misallocation of an endogenous signalling ligand rather than from conventional gain- or loss-of-function mutations in downstream signalling components.

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BibTeXRIS

Kim, Y., Zhao, Y., Xu, A. X., Nakhuda, A., Androniciuc, A.-M., Tenin, G., Keavney, B. D., Jones, E. Y., McGough, I. J.. 2026-09-22. GPC6 Lipid Shielding Sustains WNT5A Gradients to Prevent a RASopathy-like Developmental State. https://doi.org/10.64898/2026.09.20.752969

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