Isoleucine and asparagine influence the growth of staphylococcal strains in atopic dermatitis

Wai, H. K.-F.; Wang, X.; Li, C. C. Y.; Chua, G. T.; Wong, C. S. M.; Rosa Duque, J. S.; Chan, V. W. Y.; Le Guennec, A.; Zhu, J.; Khan, S.; Punet, A. N.; Ji, M.; Zhang, J.; Hill, E. S.; Che, Y.; Alexander, H.; Deharo, E.; Tun, H. M.; Moyes, D. L.

doi:10.64898/2026.09.15.751654

bioRxiv · 10.64898/2026.09.15.751654

Isoleucine and asparagine influence the growth of staphylococcal strains in atopic dermatitis

Wai, H. K.-F.·Wang, X.·Li, C. C. Y.·Chua, G. T.·Wong, C. S. M.·Rosa Duque, J. S.·Chan, V. W. Y.·Le Guennec, A.·Zhu, J.·Khan, S.·Punet, A. N.·Ji, M.·Zhang, J.·Hill, E. S.·Che, Y.·Alexander, H.·Deharo, E.·Tun, H. M.·Moyes, D. L.

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Abstract

Atopic dermatitis (AD) is a multifactorial, chronic skin disease associated with microbial factors. It is known that staphylococcal skin microbes play a role in the pathophysiology of AD, notably S. aureus. However, how staphylococcal strain variation affects disease aetiology and progression remains unclear. Using a culture-dependent approach, we analyzed the genomic and phenotypic profile of staphylococcal strains isolated from a Hong Kong children cohort (n = 70). Presence of S. aureus was positively associated with AD skin, whilst S. epidermidis and S. hominis were positively associated with healthy skin, but no overall strain type was associated with AD. In vitro experimentation revealed that there is demarcation in S. aureus strain growth, biofilm biomass formation, and metabolite production by disease severity. While isoleucine and asparagine promoted S. aureus growth, these amino acids suppressed S. hominis growth. These collective findings highlight the importance of strain-level and metabolic interactions of staphylococci in AD pathophysiology.

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Wai, H. K.-F., Wang, X., Li, C. C. Y., Chua, G. T., Wong, C. S. M., Rosa Duque, J. S., Chan, V. W. Y., Le Guennec, A., Zhu, J., Khan, S., Punet, A. N., Ji, M., Zhang, J., Hill, E. S., Che, Y., Alexander, H., Deharo, E., Tun, H. M., Moyes, D. L.. 2026-09-16. Isoleucine and asparagine influence the growth of staphylococcal strains in atopic dermatitis. https://doi.org/10.64898/2026.09.15.751654

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