The Pentose Phosphate Pathway Regulates Myelo-Lymphoid Lineage Specification

cosgrove, J.; Donada, A.; Lyne, A. M.; Krabacz, N.; Rodriguez-Ramiro, I.; Midoun, A.; Cabeli, V.; Conrad, C.; Jouault, G.; Durand, A.; Teinreira, S.; Tubeuf, E.; Demollin, H.; Russo, E.; Cayrac, F.; Belloucif, Y.; Maleki, S.; Reed, S.; Monaco, F.; Ruffinatto, L.; de Laval, B.; Ager, A.; lobry, c.; Bousso, P.; Fernandez, P. J.; Vallot, C.; Isambert, H.; Cabezas-Wallscheid, N.; ARGUELLO, R. J.; Perie, L.

doi:10.64898/2026.09.07.747966

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bioRxiv · 10.64898/2026.09.07.747966

The Pentose Phosphate Pathway Regulates Myelo-Lymphoid Lineage Specification

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Abstract

Following infection, hematopoietic stem and progenitor cells (HSPCs) support immunity by increasing the rate of innate immune cell production but the metabolic cues that guide this process are unknown. To address this question, we combined in situ RNA barcoding and metabolomics approaches to perform metabolic state-fate mapping in vivo. This approach revealed a subset of myeloid-biased HSPCs that express a distinct set of metabolic enzymes and transporters as well as the surface marker CD62L. Metabolically, CD62Lhigh HSPCs have differential activity of the pentose phosphate pathway (PPP), OXPHOS and translation, as well as differential levels of S-adenosylmethionine (SAM) cycle metabolites associated with epigenetic modifications. Inhibition of the PPP skews HSPC lineage fate decisions by disrupting myeloid associated enhancers, while simultaneously increasing enhancer activity for the master B-lymphoid regulator Ikaros. In vivo, overexpression of glucose-6-phosphate dehydrogenase, a rate limiting enzyme of the PPP, skewed HSPC output from B-lymphocytes. In summary, our data shows that HSPCs undergo significant metabolic changes to facilitate the bioenergetic and epigenetic demands of myeloid versus lymphoid lineage specification. We highlight a key role for the pentose phosphate pathway which modulates myeloid rather than lymphoid commitment by shaping the HSPC enhancer landscape, providing proof of principle that HSPC metabolism can be targeted to modulate immune system dynamics.

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cosgrove, J., Donada, A., Lyne, A. M., Krabacz, N., Rodriguez-Ramiro, I., Midoun, A., Cabeli, V., Conrad, C., Jouault, G., Durand, A., Teinreira, S., Tubeuf, E., Demollin, H., Russo, E., Cayrac, F., Belloucif, Y., Maleki, S., Reed, S., Monaco, F., Ruffinatto, L., de Laval, B., Ager, A., lobry, c., Bousso, P., Fernandez, P. J., Vallot, C., Isambert, H., Cabezas-Wallscheid, N., ARGUELLO, R. J., Perie, L.. 2026-09-10. The Pentose Phosphate Pathway Regulates Myelo-Lymphoid Lineage Specification. https://doi.org/10.64898/2026.09.07.747966

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