Transcriptomic differences underlying fin sexual dichromatism and male color polymorphism in bluefin killifish (Lucania goodei)

Abstract

Color polymorphisms offer a powerful lens for examining how selection shapes genetic and phenotypic variation. Genomic analyses of color variation provide deeper insight into how genetic differences and phenotypic plasticity contribute to trait variation. Here, we use the bluefin killifish (Lucania goodei) to study this relationship. Male fins can display either red or yellow coloration based on pigments (pterins) or blue structural coloration (iridophores) often induced in UV-depauperate environments. Female fins lack pigmentation. To characterize the molecular basis of this variation, we assembled a high-quality genome and examined gene expression in the anal fins of male and female bluefin killifish to identify the molecular mechanisms underlying color variation. Our gene expression analysis found sex differences driven by male-biased upregulation of melanin synthesis and canonical pigment pathway genes. We additionally identified that the progesterone sex receptor was upregulated in females. Among male color morphs, several genes involved in xanthophore and iridophore formation differed between blue and non-blue males, including the transcription factor tfec, and iridophore-patterning genes atic, pnp4a, and sox10. Extraocular opsins, which are involved in non-image-forming light perception, were expressed at low levels across all fins, but showed population- and morph-specific patterns; notably, rgrb (retinal G protein coupled receptor b) was upregulated in blue males. To the best of our knowledge, this is the first report of an extraocular opsin varying as a function of population and color morph. Together, these results identify the pigment pathways underlying male-limited color polymorphisms and reveal candidate genes likely involved in light-sensitive phenotypic plasticity.