Reduced EEG Complexity and Its Association with Social Communication in Adults with Autism Spectrum Disorder: A Multiscale Entropy Study

Abstract

Background: Brain functions emerge from temporally organized neural dynamics, and an appropriate level of neural complexity may support flexible information processing. Electroencephalographic (EEG) studies using multiscale entropy (MSE), which quantifies signal complexity across multiple temporal scales, have reported reduced MSE at longer scales in individuals with autism spectrum disorder (ASD). However, most evidence comes from studies of infant and child samples, leaving adult data scarce, and the associations between reduced MSE, clinical symptoms, and social information processing are insufficiently understood. Methods: We recorded and analyzed EEG data from adults with ASD (n = 47) and typically developing (TD) controls (n = 40) during eyes-closed rest and two movie viewing conditions: Inscapes, comprising dynamically changing abstract visual patterns, and Partly Cloudy, an emotionally engaging animated social narrative. Group and condition effects were assessed using cluster-based permutation tests, and associations between MSE and Social Affect (SA) scores from the Autism Diagnostic Observation Schedule, Second Edition (ADOS-2), as well as group differences in event-related MSE changes, were examined using linear mixed-effects models. Results: Across conditions, adults with ASD showed lower MSE at longer scales ({tau} = 16-30), corresponding to effective sampling rates of 12.50-6.67 Hz in the coarse-grained time series, than TD adults. Longer-scale MSE within the ASD-TD difference cluster was negatively associated with ADOS-2 SA scores across diagnostic groups. The difference in event-related MSE change between Social and Non-Social conditions, comparing empathic-pain and mentalizing events in Partly Cloudy with temporally matched non-social windows in Inscapes, was smaller in ASD participants than in TD participants. No significant difference between Social and Non-Social windows was observed among ASD participants, whereas TD participants showed significantly greater MSE change during Social than Non-Social windows. Limitations: This study used a modest sample without an independent replication cohort, and the short event windows limited the precision of the MSE estimation. Conclusions: Reduced longer-scale MSE extends to adults with ASD and is associated with higher ADOS-2 SA scores across diagnostic groups. Event-related findings further suggest attenuated differentiation of longer-scale MSE between socially relevant and matched non-social windows in ASD.