Placental transcriptomics reveals alterations in metabolic and transport gene programs following prenatal cannabis smoke exposure

Monaco, C.; Podinic, T.; Xhuti, D.; Feeney, J.; Tan, D.; Lemaire, M.; MacAndrew, A.; Sunil, M.; Nederveen, J. P.; Wilson, S. L.; Ratcliffe, E.; Raha, S.

doi:10.64898/2026.08.31.748443

bioRxiv · 10.64898/2026.08.31.748443

Placental transcriptomics reveals alterations in metabolic and transport gene programs following prenatal cannabis smoke exposure

Monaco, C.·Podinic, T.·Xhuti, D.·Feeney, J.·Tan, D.·Lemaire, M.·MacAndrew, A.·Sunil, M.·Nederveen, J. P.·Wilson, S. L.·Ratcliffe, E.·Raha, S.

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Abstract

Background: Prenatal cannabis use is increasing, yet the effects of inhaled cannabis smoke on placental molecular function remain poorly defined. Objective: To characterize placental transcriptomic alterations following prenatal cannabis smoke exposure using a physiologically relevant murine inhalation model. Methods: Timed-pregnant CD-1 mice were exposed to cannabis cigarette smoke or room air from embryonic day (E)6.5 to 18.5. Placentae were collected at E18.5 for microarray-based transcriptomic profiling. Differential gene expression was assessed using fetal sex adjusted linear models and false discovery rate correction. Gene set variation and ranked pathway enrichment were performed to evaluate compartment-associated and biological transcriptional programs. Selected genes were validated by quantitative PCR. Results: Large-scale transcriptomic profiles revealed no clear visual clustering by exposure; however, 23 genes were differentially expressed (FDR <0.05, |log2 fold change| > 0.5). Labyrinth-associated transcriptional signatures were reduced, whereas junctional-associated signatures were increased in exposed placentae. Pathway analyses revealed negative enrichment of fatty acid metabolism, bioenergetic, and transmembrane transport pathways, alongside positive enrichment of RNA processing and transcriptional-regulatory pathways. Quantitative PCR confirmed altered expression of multiple transport-related genes. Electron transport chain-associated gene expression was altered despite preserved placental respiratory measurements. Conclusions: Prenatal cannabis smoke exposure is associated with subtle yet coordinated placental transcriptional alterations involving metabolic, transport-associated, and gene-regulatory pathways, alongside compartment-associated transcriptional shifts. These findings provide insight into placental responses to whole cannabis smoke exposure and identify pathways that may contribute to altered placental function during pregnancy.

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Monaco, C., Podinic, T., Xhuti, D., Feeney, J., Tan, D., Lemaire, M., MacAndrew, A., Sunil, M., Nederveen, J. P., Wilson, S. L., Ratcliffe, E., Raha, S.. 2026-09-04. Placental transcriptomics reveals alterations in metabolic and transport gene programs following prenatal cannabis smoke exposure. https://doi.org/10.64898/2026.08.31.748443

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