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bioRxiv · 10.64898/2026.08.24.746705

A novel approach to incorporate compound dissimilarity to plant chemodiversity measures using UV-Vis spectra

Aragam, K. S.·Steppuhn, A.
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Abstract

1. Since plants interact with their environment through complex combinations of phytochemicals rather than metabolites in isolation, quantifying plant chemodiversity is of increasing interest. Although functionally relevant, structural disparity is mostly neglected in measures of chemodiversity because its integration relies on known compound identity, limiting broad application. 2. We established an approach deriving compound dissimilarity from UV-Vis spectra in HPLC-DAD datasets, evaluated how it relates to structure- or biosynthesis-based approaches, and examined whether it provides meaningful contributions to chemodiversity measures. For this, we applied it in an experiment full-factorially testing the effects of drought and herbivory on Solanum dulcamara leaf chemodiversity. 3. UV-Vis spectral dissimilarity aligned well with fMCS-based structural dissimilarity and reflected structural relationships within a set of standards. Incorporating it into chemodiversity analysis improved separation of the effects of drought and herbivory. Especially in the combination of both stresses, their distinct effects on different leaf metabolites were only fully reflected when accounting for compound disparity. 4. Hence, UV-Vis spectral dissimilarity captures chemically meaningful compound relatedness without requiring compound identity. Using it as a proxy for compound disparity adds a biologically relevant dimension to measures of chemodiversity with broader implications when assessing functional consequences of phytochemical diversity.

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Aragam, K. S., Steppuhn, A.. 2026-08-25. A novel approach to incorporate compound dissimilarity to plant chemodiversity measures using UV-Vis spectra. https://doi.org/10.64898/2026.08.24.746705

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