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bioRxiv · 10.64898/2026.08.21.746263

Chromatin compartments define distinct modes of action and biological roles of the pioneer Pax7

Gouhier, A.·Harris, J.·Lapointe-Roberge, V.·Marcil, A.·Balsalobre, A.·DROUIN, J.
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Abstract

Pioneer transcription factors direct cell differentiation by remodeling closed chromatin to deploy new enhancers. It remains unclear how the chromatin context shapes their action. During pituitary development, the pioneer PAX7 opens thousands of enhancers and specifies the melanotrope cell fate. We report that PAX7 acts in both the A (active) and B (inactive) genomic compartments, engaging two distinct closed states: H1-enriched chromatin in A, and H3K9me2- and lamin-rich chromatin in B. Whereas A-compartment enhancers open in a single, cell-division-independent step, B-compartment opening requires cell division and triggers domain-wide lamin dissociation coupled with B-to-A compartment shift. The two modes involve distinct biological outputs: A-compartment action modulates broadly expressed genes, whereas B-compartment action drives de novo expression of cell-type-specific developmental genes central to melanotrope identity.

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Gouhier, A., Harris, J., Lapointe-Roberge, V., Marcil, A., Balsalobre, A., DROUIN, J.. 2026-08-25. Chromatin compartments define distinct modes of action and biological roles of the pioneer Pax7. https://doi.org/10.64898/2026.08.21.746263

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