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bioRxiv · 10.64898/2026.08.17.745250

No-code microbial growth phenotyping with GUIbiont

Alvarenga, E. Z.·Oltolini, E.·Pinheiro, F.
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Abstract

Microbial growth screens generate thousands of curves, but cross-experiment comparison and mapping growth phenotypes to genotypes or environments routinely require custom code. GUIbiont is a no-code browser application for quality control, curve fitting, clustering and metadata-linked analysis with machine learning techniques. Interactive sessions export as Julia scripts, allowing users to reproduce or extend browser analyses. Validated across 3,885 E. coli deletion strains and 13,608 defined-media curves, GUIbiont recovered known auxotrophic and nutrient-dependent phenotypes.

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Alvarenga, E. Z., Oltolini, E., Pinheiro, F.. 2026-08-21. No-code microbial growth phenotyping with GUIbiont. https://doi.org/10.64898/2026.08.17.745250

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