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bioRxiv · 10.64898/2026.08.11.744301

The engagement of layer 6 corticothalamic neurons in somatosensation

Dimwamwa, E. D.·Chang, N. H.·Waiblinger, C.·Stanley, G. B.
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Abstract

The corticothalamic neurons from layer 6 (L6CT) of primary sensory cortices provide extensive input to the thalamus in addition to projecting within the cortex, positioning them to play a key hypothesized role in shaping thalamocortical signaling. With the expansion of tools for precise functional identification of L6CT neurons for in-vivo electrophysiology, increasing evidence highlights L6CT neurons as dynamic gain modulators of thalamocortical sensory responses. However much of the work to date has been conducted under anesthesia and not in the context of awake and/or behaving animals. In this study, we show that L6CT neurons in the awake mouse convey information about ascending sensory inputs, the timing of which is fast enough to contribute to the sensory response of neurons throughout the thalamocortical circuit. Overall, L6CT neurons robustly encode the presence vs absence of a sensory stimulus but are relatively weak encoders of the fine details. Benchmarked against the activity of other excitatory cortical neuron, we also provide evidence for L6CT neurons as predictors of the behavioral outcome during a trained detection task. Taken together, the results in this study tie L6CT neurons to behavior in tactile detection, one of the most fundamental functional roles of the pathway.

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Dimwamwa, E. D., Chang, N. H., Waiblinger, C., Stanley, G. B.. 2026-08-20. The engagement of layer 6 corticothalamic neurons in somatosensation. https://doi.org/10.64898/2026.08.11.744301

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