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bioRxiv · 10.64898/2026.07.31.741637

Asthma Exacerbations: Integrative Analysis of miRNA Activity Using Single-Cell Transcriptomics

Hadikhani, P.·Yan, X.·Chupp, G. L.·Ban, G. Y.·Piparia, S.·McGeachie, M.·Sharma, R.·Weiss, S. T.·Laurent, L. C.·Kho, A. T.·Tantisira, K. G.
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Abstract

BackgroundAsthma exacerbations are caused by dysregulated cellular interactions between airway and immune cell populations. Circulating microRNAs (miRNAs) are potential biomarkers for asthma exacerbations; however, their target airway cells remain poorly defined. ObjectiveTo identify the cell types that are regulated by the circulating microRNAs linked to asthma exacerbations and the extent to which the cells are regulated by miRNAs. MethodsWe integrated a curated panel of exacerbation-associated circulating miRNAs with single-cell RNA sequencing (scRNA-seq) profiles from induced sputum of 16 asthma patients and 8 healthy controls. Experimentally validated miRNA-target interactions were combined with cell-type-specific differential expression. Elastic Net regression and SHAP analysis quantified gene-level regulatory contributions, yielding a composite Regulation Strength metric. Findings were validated against four independent GEO datasets. ResultsImmune cells, including monocytes, dendritic cells, and macrophages, demonstrated the strongest statistically significant miRNA regulatory signals, in contrast to airway epithelial cells.hsa-miR-222-3p showed opposing regulatory effects in mature versus alveolar macrophages, indicating differentiation-state-dependent activity, while B_Plasma cells showed no detectable regulatory effect from any miRNA tested. Independent GEO validation confirmed higher expression of protective miRNAs (hsa-miR-126-3p, hsa-miR-146b-5p) in healthy individuals, consistent with prior CAMP cohort associations. ConclusionCirculating miRNAs show cell-type-specific regulatory activity, strongest in monocytes, dendritic cells, and macrophages. hsa-miR-222-3p showed opposing regulatory directions between macrophage subtypes, while B_Plasma cells showed no effect, validated across independent GEO cohorts.

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Hadikhani, P., Yan, X., Chupp, G. L., Ban, G. Y., Piparia, S., McGeachie, M., Sharma, R., Weiss, S. T., Laurent, L. C., Kho, A. T., Tantisira, K. G.. 2026-08-06. Asthma Exacerbations: Integrative Analysis of miRNA Activity Using Single-Cell Transcriptomics. https://doi.org/10.64898/2026.07.31.741637

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