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bioRxiv · 10.64898/2026.07.29.741476

Plug-and-Play 3D localization microscopy

Cohen, O. R.·Xiao, D.·Kedem, R. O.·ALALOUF, O.·Prakash, J.·NAKATANI, Y.·Gustavsson, A.-K.·Shechtman, Y.
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Abstract

Point-spread-function engineering by depth-encoding phase masks enables volumetric super-resolution imaging by 3D single-molecule localization microscopy (SMLM) but usually requires cumbersome relay optics. We demonstrate simple 3D SMLM implementation by phase mask insertion directly into the infinity space of a commercial microscope, along with appropriate computational compensation for field-dependence. The entire inserted component, including the phase mask, is 3D printing-based.

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Cohen, O. R., Xiao, D., Kedem, R. O., ALALOUF, O., Prakash, J., NAKATANI, Y., Gustavsson, A.-K., Shechtman, Y.. 2026-07-30. Plug-and-Play 3D localization microscopy. https://doi.org/10.64898/2026.07.29.741476

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