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bioRxiv · 10.64898/2026.07.25.740666

The Mechanical and Biological Evolution of Pressure Ulcer Formation and Healing in Mice

Lin, C.-Y.·Sreedhar, S.·Lohr, M. J.·Kostelnik, C. J.·Madariaga, A.·Tepole, A. B.·Rausch, M. K.
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Abstract

Pressure ulcers arise from sustained mechanical loading that impairs perfusion and damages skin tissues, yet the coupled mechanical and biological mechanisms of their formation and healing remain poorly characterized. We addressed this gap using a mouse model in which dorsal skin underwent 72 hours of magnet-induced ischemia followed by reperfusion, with tissue collected at 0, 3, 6, and 9 days and compared with baseline controls. From each mouse, we obtained paired samples from pressure ulcer and remote control (non-loaded) sites, mapped thickness by tissue profilometry, and performed equibiaxial testing with full-field digital image correlation and inverse finite element analysis to estimate regional material parameters. In parallel, we quantified CD31+ vasculature, F4/80+ macrophages, collagen content, and key cytokines. Pressure ulcer sites were compressed and thinner at Day 0, developed ulcers by Day 3, and continued to remodel through Day 9. Mechanical tests revealed heterogeneous strain fields with elevated deformation along ulcer borders, while remote control tissue deformed more homogeneously. These mechanical changes evolved alongside dynamic vessel and macrophage repopulation, increased collagen content at early time points, and cytokine upregulation within pressure ulcer tissue. Collectively, our data define the spatiotemporal co-evolution of tissue geometry, mechanics, collagen remodeling, and inflammation in pressure ulcers and provide a quantitative foundation for predictive mechanobiological models.

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Lin, C.-Y., Sreedhar, S., Lohr, M. J., Kostelnik, C. J., Madariaga, A., Tepole, A. B., Rausch, M. K.. 2026-07-27. The Mechanical and Biological Evolution of Pressure Ulcer Formation and Healing in Mice. https://doi.org/10.64898/2026.07.25.740666

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