Palliative Irradiation Affects Temporal Degradation of Rodent Vertebral Bone Mechanics, Architecture, and Composition

Wang, C.; Berardi, M.; Martin, S.; Brown, C.; Soltani, Z.; Keko, M.; Rosa-Caldwell, M. E.; Mortreux, M.; Rutkove, S.; Bailey, S.; Alkalay, R. A.

doi:10.64898/2026.07.14.738210

bioRxiv · 10.64898/2026.07.14.738210

Palliative Irradiation Affects Temporal Degradation of Rodent Vertebral Bone Mechanics, Architecture, and Composition

Wang, C.·Berardi, M.·Martin, S.·Brown, C.·Soltani, Z.·Keko, M.·Rosa-Caldwell, M. E.·Mortreux, M.·Rutkove, S.·Bailey, S.·Alkalay, R. A.

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Abstract

BackgroundPalliative radiation therapy (RT) for metastatic spine disease significantly increases the risk of vertebral fractures. However, the temporal mechanisms underlying radiation-induced vertebral bone fragility remain poorly understood. ObjectiveTo evaluate the longitudinal effects of a single high-dose irradiation, simulating palliative RT, on vertebral bone mechanical, architectural, and compositional properties in a healthy, skeletally mature rat model. MethodsThirty-one male Sprague Dawley rats received a single 15 Gy lumbar spine irradiation (IR). L4 vertebrae were assessed across all groups (irradiation: 7, 14, and 28 days post-IR, controls: at 0 and 28 days post-IR) for compressive strength and stiffness, micro-CT-derived bone composition and trabecular indices, serum bone turnover markers (NTX and BAP) and advanced glycation endproducts (AGEs). ResultsIrradiation induced progressive deterioration of vertebral bone mechanical properties, with strength decreasing up to 44% and stiffness up to 38% by 28 days post-IR, compared to 0- day controls. Trabecular bone exhibited reduced BMD, BV/TV, and Tb.N with increased Tb.Sp, a shift toward a more rod-like structure. Early post-IR changes suggested disrupted bone remodeling, characterized by elevated NTX and AGEs, but decreased BAP. Multivariable regression demonstrated that Tb.Th and AGEs were independent predictors of stiffness, collectively explaining 61% of its variance. DiscussionHigh-dose irradiation induces sustained temporal degradation of vertebral mechanical properties driven by both trabecular architectural deterioration and alterations in bone matrix quality. Measures of bone composition and non-enzymatic bone turnover suggest this early damage was driven by disruption of bone cellular homeostasis, favoring increased resorption over formation. These findings support that radiation impairs both structural integrity and pre-yield mechanical behavior, providing mechanistic insight into the elevated fracture risk observed clinically after irradiation for metastatic spine disease. Lay summaryThis study used a rat model to mimic palliative radiation therapy for cancer that has spread to the spine and evaluated the changes in bone quality up to 28 days post-therapy. We found that irradiation progressively weakened the structural integrity and composition of the bones in the spine and disrupted the normal balance of bone breakdown and repair, leading to greater bone loss and fragility. Our findings provide insight into the increased risk of fractures observed in patients receiving radiation therapy to the spine and may support efforts to better protect bone health during treatment.

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Wang, C., Berardi, M., Martin, S., Brown, C., Soltani, Z., Keko, M., Rosa-Caldwell, M. E., Mortreux, M., Rutkove, S., Bailey, S., Alkalay, R. A.. 2026-07-15. Palliative Irradiation Affects Temporal Degradation of Rodent Vertebral Bone Mechanics, Architecture, and Composition. https://doi.org/10.64898/2026.07.14.738210

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