A large-scale analysis of the R2TP chaperone network reveals its contribution to the assembly of INO80, SRCAP and TIP60

Abel, Y.; Philippe, M.; Decourty, L.; Paiva, A. C. F.; Busse, P.; Robert, M.-c.; Urbach, S.; Bellieres, C.; Vandermoere, F.; Imbert, J.; Seveno, M.; Saveanu, C.; Sousa, P.; Boulon, S.; Bandeiras, T.; Bertrand, E.; Verheggen, C.

doi:10.64898/2026.07.07.737026

bioRxiv · 10.64898/2026.07.07.737026

A large-scale analysis of the R2TP chaperone network reveals its contribution to the assembly of INO80, SRCAP and TIP60

Abel, Y.·Philippe, M.·Decourty, L.·Paiva, A. C. F.·Busse, P.·Robert, M.-c.·Urbach, S.·Bellieres, C.·Vandermoere, F.·Imbert, J.·Seveno, M.·Saveanu, C.·Sousa, P.·Boulon, S.·Bandeiras, T.·Bertrand, E.·Verheggen, C.

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Abstract

HSP90/R2TP is an essential quaternary chaperone composed of RPAP3, PIH1D1 and the RUVBL1/RUVBL2 AAA+ ATPases. These enzymes are also part of the INO80, SRCAP and TIP60 complexes, but the relationship between these chromatin remodelers and R2TP remains unclear. Here, we performed systematic analyses of the R2TP-specific subunits RPAP3 and PIH1D1. We validated 115 interaction partners and found that many were sensitive to HSP90 or R2TP inhibition. In yeast, epistatic screens revealed functional interactions with Ino80, Swr1 (SRCAP) and NuA4 (TIP60). Consistently, human RPAP3 physically interacted with subunits of INO80, SRCAP and TIP60 and was required for the formation of these complexes. More specifically, RPAP3 enabled the co-translational association of RUVBL1/RUVBL2 with the motor subunit of these chromatin remodelers. In vitro, the client-binding domain of RUVBL1/RUVBL2 modulated their interaction with RPAP3, suggesting that client subunits displace RPAP3 from nascent complexes. Thus, R2TP is an early chaperone of TIP60, SRCAP and INO80, which leaves RUVBL1/RUVBL2 as resident scaffolding subunits.

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Abel, Y., Philippe, M., Decourty, L., Paiva, A. C. F., Busse, P., Robert, M.-c., Urbach, S., Bellieres, C., Vandermoere, F., Imbert, J., Seveno, M., Saveanu, C., Sousa, P., Boulon, S., Bandeiras, T., Bertrand, E., Verheggen, C.. 2026-07-08. A large-scale analysis of the R2TP chaperone network reveals its contribution to the assembly of INO80, SRCAP and TIP60. https://doi.org/10.64898/2026.07.07.737026

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