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bioRxiv · 10.64898/2026.06.30.735497

Transforming avian to mammalian modes of digit patterning

Abstract

How a conserved embryonic organiser--the Sonic hedgehog (Shh)-expressing Zone of Polarising Activity (ZPA)--generates diverse limb architectures in amniotes remains a central problem in evolutionary and developmental biology. The principal difference across species lies in the number of digits produced from ZPA tissue: two in mammals1, one in the chick leg2 and none in the chick wing2. Here we show that this divergence is governed by a Shh-p27Kip1 pathway operating in avian, but not mammalian ZPAs. In chick wing ZPA explants, attenuation of this pathway reveals an intrinsic digit-forming programme, enabling cells to self-organise signalling networks and generate digits after grafting into a host wing bud. Guided by these findings, we redirected the chick leg, which retains stem amniote digit identities, to follow a mammalian-like developmental trajectory. Precise temporal restriction of Shh signalling transforms chick legs into pentadactyl limbs with digit identities characteristic of mammals and their therapsid ancestors, with two digits arising from the ZPA. These findings establish a unifying framework for how Shh controls both digit number and identity across amniotes.

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BibTeXRIS

Wiggins, S., Perez, S. S., Placzek, M., Cooper, R., Towers, M.. 2026-07-01. Transforming avian to mammalian modes of digit patterning. https://doi.org/10.64898/2026.06.30.735497

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