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bioRxiv · 10.64898/2026.06.26.732348

MCD Stitcher: An open-source tool for whole-slide stitching and conversion of Imaging Mass Cytometry data

Chaurasia, P.
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Abstract

Imaging Mass Cytometry (IMC) combines metal-tagged antibody labelling with laser ablation mass spectrometry to generate highly multiplexed spatial images of tissue sections. However, the area that can be acquired within a single region of interest (ROI) is limited by hardware and software constraints, requiring large tissues to be imaged as multiple tiled ROIs. Reconstructing these ROIs into whole-slide images requires additional processing, while the proprietary .mcd file format can hinder integration with standard bioimage analysis workflows. Here, we present MCD Stitcher, an open-source Python package for converting .mcd files into OME-TIFF images with automated whole-slide stitching. The tool supports rectangular and polygonal ROIs, accommodates variable pixel sizes between ROIs, and uses memory-aware chunked reading during data ingestion to process large datasets on standard workstations. The generated OME-TIFF outputs preserve spatial, channel, and acquisition metadata for downstream analysis in tools such as QuPath, napari, and ImageJ/Fiji. MCD Stitcher provides a reproducible workflow for converting raw IMC data into interoperable image formats, enabling whole-slide spatial analysis without reliance on vendor-specific software.

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Chaurasia, P.. 2026-07-01. MCD Stitcher: An open-source tool for whole-slide stitching and conversion of Imaging Mass Cytometry data. https://doi.org/10.64898/2026.06.26.732348

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