Retinal resuscitation in post-mortem eyes

Byrne, E. M.; Di Vicino, U.; Farah, N.; Cadevall Angles, M.; Fernandez Merino, M.; Rotkevich, M.; Caravaca Rodriguez, D.; Balashov, M.; Guerra Solano, J. M.; Ibrahim, M. S.; Marin Figuera, J. A.; Puig Galy, J. J.; Sandiumenge, A.; Casaroli Marano, R. P.; Lee, J.; Mandel, Y.; Cosma, M. P.

doi:10.64898/2026.06.25.733416

bioRxiv · 10.64898/2026.06.25.733416

Retinal resuscitation in post-mortem eyes

Byrne, E. M.·Di Vicino, U.·Farah, N.·Cadevall Angles, M.·Fernandez Merino, M.·Rotkevich, M.·Caravaca Rodriguez, D.·Balashov, M.·Guerra Solano, J. M.·Ibrahim, M. S.·Marin Figuera, J. A.·Puig Galy, J. J.·Sandiumenge, A.·Casaroli Marano, R. P.·Lee, J.·Mandel, Y.·Cosma, M. P.

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Abstract

Vision loss compromises the quality of life of millions of people worldwide. Currently, vision-restoring therapies are lacking. Post-mortem preservation of human eyes may enable whole eye transplantation (WET) or therapeutic development. We developed an approach to cannulate the ophthalmic artery and perfuse post-mortem pig and human eyes using a custom-built device, Eye-in-Care-Box (ECaBox). Retinal vasculature imaging and reconstruction were used to assess perfusion efficacy using a supervised deep learning model. Without intervention, the retina degrades post-mortem, whereas perfusion preserves retinal structure and cell viability for up to 24-hours. Eyes were perfused within 30 minutes post-extraction, and regained light-responses persisted for up to 10 hours after death, challenging the notion that response to light ends at death. Our findings show that intact eyes can be resuscitated and preserved outside the body, supporting the ECaBox platform for WET preservation and pre-clinical therapeutic testing. Ultimately, ECaBox may bring vision-restoring treatments to patients.

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Byrne, E. M., Di Vicino, U., Farah, N., Cadevall Angles, M., Fernandez Merino, M., Rotkevich, M., Caravaca Rodriguez, D., Balashov, M., Guerra Solano, J. M., Ibrahim, M. S., Marin Figuera, J. A., Puig Galy, J. J., Sandiumenge, A., Casaroli Marano, R. P., Lee, J., Mandel, Y., Cosma, M. P.. 2026-06-30. Retinal resuscitation in post-mortem eyes. https://doi.org/10.64898/2026.06.25.733416

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