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bioRxiv · 10.64898/2026.06.17.732826

Microbial community-based single-cell protein supports partial fishmeal replacement in juvenile Asian seabass diets across feeding trials and production scales

Santillan, E.·Loo, P. L.·Yasumaru, F.·Xu, H.·Neshat, S. A.·Vethathirri, R. S.·Zhou, Y.·Chan, D.·Wuertz, S.
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Abstract

The growing demand for sustainable aquafeeds has intensified interest in alternative protein ingredients capable of reducing reliance on fishmeal without compromising fish performance. Here, we evaluated microbial community-based single-cell protein (SCP) as a fishmeal substitute in juvenile Asian seabass (Lates calcarifer) diets in two independent feeding trials of juvenile fish conducted over 49 and 56 days, respectively and compared them to a previous study that lasted 24 days. SCP was produced from nutrient-rich soybean-processing side streams by microbial communities in fermenters and incorporated into experimental diets at inclusion levels ranging from 10% to 100% fishmeal replacement. In the 24-day trial, a diet containing 50% fishmeal replacement with lab-scale produced SCP achieved 100% survival and a feed conversion ratio (FCR), specific growth rate (SGR), and weight gain comparable to the fishmeal control diet. In the 49-day trial using pilot-scale produced SCP, a 50% fishmeal replacement also maintained an FCR and feed intake comparable to the control, whereas complete replacement reduced feed intake and growth performance. In a 56-day pilot-scale trial that used 500-L fish tanks, diets containing up to 50% fishmeal replacement maintained comparable survival, weight gain, and SGR, although moderately higher FCR values were observed at higher SCP inclusion levels. Proximate composition and essential amino acid profiles of fish fed control or SCP-containing diets were comparable. Genome-resolved metagenomic analyses revealed diverse microbial taxa associated with the SCP. Collectively, these findings support microbial community-based SCP as a scalable and reproducible alternative protein platform for aquaculture feeds across independent trials and production scales.

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Santillan, E., Loo, P. L., Yasumaru, F., Xu, H., Neshat, S. A., Vethathirri, R. S., Zhou, Y., Chan, D., Wuertz, S.. 2026-06-17. Microbial community-based single-cell protein supports partial fishmeal replacement in juvenile Asian seabass diets across feeding trials and production scales. https://doi.org/10.64898/2026.06.17.732826

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