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bioRxiv · 10.64898/2026.06.12.731929

The Logic of Thalamic Inputs onto the Molecular Taxonomy of Cortical Neurons Reveals a Visual Hierarchy

Ge, X.·Wei, X.·Ruan, B.·Wu, Q. Y.·Chang, S.·Tsai, N.·Zhang, S.·Duan, X.·Scanziani, M.
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Abstract

The hierarchical organization of sensory cortices and the rich molecular taxonomy of their cell types are defining features of the mammalian cortex. Cortical areas along this hierarchy are reciprocally connected via the thalamus through bottom-up and top-down projections. The logic through which these projections map onto the cellular taxonomy of the cortex is, however, poorly understood. Here we combine an anterograde transsynaptic tracer with spatial transcriptomics to reveal the molecular and spatial identity of mouse visual cortical neurons downstream of thalamic starter neurons across visual cortical areas. Distinct thalamic inputs target characteristic sets of molecularly defined cortical neurons, forming bottom-up or top-down "signatures" defined by cell-type composition and the ratio of GABAergic to glutamatergic neurons. These signatures reveal a hierarchy spanning thalamic nuclei and visual cortical areas, independently predicted by the molecular and cellular similarities between cortical areas. This work uncovers basic principles of how bottom-up and top-down thalamic inputs map onto the cellular taxonomy in the visual cortex and establishes a cellular framework for the cortical hierarchy.

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Ge, X., Wei, X., Ruan, B., Wu, Q. Y., Chang, S., Tsai, N., Zhang, S., Duan, X., Scanziani, M.. 2026-06-13. The Logic of Thalamic Inputs onto the Molecular Taxonomy of Cortical Neurons Reveals a Visual Hierarchy. https://doi.org/10.64898/2026.06.12.731929

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