Freshwater input and tidal position regulate species turnover and interaction rewiring in intertidal ecological networks

Abstract

The effect that environmental conditions have on community and network assembly processes remains unclear, in part because these processes operate at multiple scales. Because marine primary producers and microinvertebrates have limited mobility, are susceptible to multiple stressors, and can be observed interacting in situ, their habitat-based interactions provide an informative system for disentangling network organising processes. We sampled 646 habitat-use networks, quantifying interactions involving habitat-users and biogenic habitat-formers over 12 months at 9 sites within Te Ihutai/Avon-Heathcote estuary in Christchurch, Aotearoa New Zealand. Using generalised dissimilarity mixed-effect models, we examined whether changes to species interactions - deconstructed into species turnover and interaction rewiring - were modulated by environmental covariates, including freshwater discharge, elevation, temperature, spatial location and season. We found that with increasing dissimilarity in sites proximity to freshwater, interaction change was more driven by rewiring, whereas differences in elevation (i.e., between channels and non-channel habitats) were driven by species turnover, with more sessile species inhabiting tidal channels. The proximity of habitats also played a strong role, with nearby networks comprising more similar interactions, and species turnover becoming more prevalent with increasing distance. Our results highlight that the relative influence and magnitude of rewiring and species turnover in controlling estuarine interaction networks was affected by the individual species distributions across the estuary and their responses to separate, but co-occurring, environmental factors. Quantification of habitat-former/user interaction networks offers robust, albeit understudied, measures of processes that can underpin community assembly, highlighting their potential importance in research, management and conservation. Open research statementData are provided for peer review. The code for produced from the project analysis and used to draft this manuscript is shared via a public GitHub repository hosted by the Tonkin Research group (repo name: EstInteractTurn). The data used in the formal analysis is hosted on Zenodo, under the corresponding authors profile (doi: 10.5281/zenodo.20619091). The data and code was prepared following strict adherence to the FAIR principles, meaning all data was saved as comma-separated values (.csv) or native R data structures (.rds).