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bioRxiv · 10.64898/2026.06.05.730314

Metadata Collector: An Open-Source Platform for Standardized Metadata Management in Multi Centre Sequencing Projects

Liguori, R.·Ferrazzi, F.
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Abstract

BackgroundNext-generation sequencing (NGS) projects generate increasingly complex metadata that are critical for reproducibility, interoperability, and compliance with FAIR principles. Nevertheless, metadata curation in multi-institutional settings often still relies on spreadsheets, manual data entry and curation, as well as non-standardized terminology. These practices frequently result in incomplete or inconsistent annotations, hinder metadata sharing, and delay submission to public repositories. ResultsWe developed Metadata Collector as a React/API/PostgreSQL web platform and deployed it on a Kubernetes cluster within a large German research consortium. The platform implements a flexible, machine-readable metadata model for experimental data and integrates customizable templates, controlled vocabularies designed to support future ontology integration, and a complete event-based versioning model. Since deployment, Metadata Collector has been used across 32 projects involving RNA-seq, scRNA-seq, ATAC-seq and multiomics datasets, representing over 700 annotated samples contributed by multiple consortium partners. The platform is designed for use by non-computational researchers as well as centralized facilities and can be integrated into existing research data management infrastructures. ConclusionsMetadata Collector embeds standardization early in the metadata lifecycle, ensuring consistent, FAIR-aligned, and reproducible metadata across distributed research groups. Its modular, open-source architecture supports both local and consortium-scale deployments and provides a foundation for future extensions, including multi-omics support and integration with laboratory information management systems and automated submission pipelines. AvailabilityOpen-source under MIT license: https://github.com/spang-lab/metadata-collector Graphical abstract of Metadata Collector O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=150 SRC="FIGDIR/small/730314v1_ufig1.gif" ALT="Figure 1"> View larger version (63K): org.highwire.dtl.DTLVardef@c7e913org.highwire.dtl.DTLVardef@9701acorg.highwire.dtl.DTLVardef@1eed3eborg.highwire.dtl.DTLVardef@9af69a_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Liguori, R., Ferrazzi, F.. 2026-06-07. Metadata Collector: An Open-Source Platform for Standardized Metadata Management in Multi Centre Sequencing Projects. https://doi.org/10.64898/2026.06.05.730314

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