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bioRxiv · 10.64898/2026.06.01.729054

Real-time Four-dimensional Imaging and Flow Dynamics Analysis of Cilia-driven Transport in Mammalian Tissues

Porcella, G.·Sahin, A. T.·Keller, J.·Nawroth, J.
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Abstract

Quantifying cilia-driven transport in mammalian tissues requires resolving rapid ciliary dynamics while preserving native three-dimensional geometry. Here we combine real-time four-dimensional light-field microscopy with tomographic particle tracking and physics-informed volumetric flow reconstruction to simultaneously image ciliary activity and reconstruct three-dimensional velocity fields in minimally dissected tissues. Applied across brain, respiratory, and reproductive epithelia, the workflow reveals conserved flow structures across individual and enables quantitative analysis of flow-tissue coupling.

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Porcella, G., Sahin, A. T., Keller, J., Nawroth, J.. 2026-06-02. Real-time Four-dimensional Imaging and Flow Dynamics Analysis of Cilia-driven Transport in Mammalian Tissues. https://doi.org/10.64898/2026.06.01.729054

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