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bioRxiv · 10.64898/2026.05.30.728955

LFCT: A Benchmark Dataset for Low-Frame-Rate Cell Tracking in Long-Term Live-Cell Microscopy

Gachloo, M.·Biswas, T.·Lu, X.·Greene, C. M.·Hargett, C. K.·Simancik, K. R.·Birtwistle, M. R.·Iuricich, F.
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Abstract

Cell tracking in time-lapse microscopy is essential for studying dynamic biological processes such as migration, proliferation, and lineage formation. Existing benchmarks primarily focus on high-framerate imaging, where short temporal intervals simplify correspondence between cells across consecutive frames. We present the Low Frame-rate Cell Tracking dataset (LCFT), a benchmark dataset designed specifically for evaluating cell tracking methods under low-frame-rate conditions. The dataset contains multi-day live-cell microscopy sequences from four human cell lines (MCF10A, MDA-MB-231, HEK293T, and U87), acquired at 10x and 20x magnifications using phase-contrast and fluorescence imaging (nucleus). Ground-truth annotations include cell identifications, temporal linking, lineage relationships, and mitosis events. To generate reliable annotations, automated segmentation and tracking were combined with extensive manual curation. LCFT provides a comprehensive resource for developing and benchmarking robust cell tracking algorithms capable of handling sparse temporal sampling and large inter-frame motion in long-term live-cell imaging experiments.

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Gachloo, M., Biswas, T., Lu, X., Greene, C. M., Hargett, C. K., Simancik, K. R., Birtwistle, M. R., Iuricich, F.. 2026-06-03. LFCT: A Benchmark Dataset for Low-Frame-Rate Cell Tracking in Long-Term Live-Cell Microscopy. https://doi.org/10.64898/2026.05.30.728955

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