The CCCH-type zinc-finger PfMD3 promotes translation for malaria parasite transmission

Van Biljon, R.; Bhatnagar, S.; Kumar, R.; Gliozzi, M. L.; Xu, G.; Tripathi, A.; Ashrafi, A.; Swearingen, K.; Sinnis, P.; Llinas, M.; Painter, H. J.

doi:10.64898/2026.05.27.726058

bioRxiv · 10.64898/2026.05.27.726058

The CCCH-type zinc-finger PfMD3 promotes translation for malaria parasite transmission

Van Biljon, R.·Bhatnagar, S.·Kumar, R.·Gliozzi, M. L.·Xu, G.·Tripathi, A.·Ashrafi, A.·Swearingen, K.·Sinnis, P.·Llinas, M.·Painter, H. J.

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Abstract

Sexual maturation and fertility of the malaria causing parasite, Plasmodium spp., is critical to transmission between hosts. This developmental process involves an intricate gene expression regulatory network that drives gametocyte commitment, development, and the production of male and female gametes. Here, we characterized the role of the CCCH-type zinc-finger protein Plasmodium falciparum Male Development 3 (PfMD3) in the formation of gametes. Genetic disruption of PfMD3 revealed a male fertility defect that decreased infectivity to mosquitoes. Molecular characterization of PfMD3 revealed its binding to mRNA transcripts associated with male gamete development through a specific RNA sequence. Gametocyte proteins encoded by these PfMD3-bound transcripts decreased significantly in PfMD3 knockout parasites, suggesting that PfMD3 impacts translation. In addition to RNA-binding, PfMD3 also interacts with proteins involved in mRNA processing and nuclear export that influence translational regulation. This study establishes PfMD3 as an RNA-binding protein that impacts lineage-specific translation and male gamete fertility.

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Van Biljon, R., Bhatnagar, S., Kumar, R., Gliozzi, M. L., Xu, G., Tripathi, A., Ashrafi, A., Swearingen, K., Sinnis, P., Llinas, M., Painter, H. J.. 2026-05-28. The CCCH-type zinc-finger PfMD3 promotes translation for malaria parasite transmission. https://doi.org/10.64898/2026.05.27.726058

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