Search bioRxiv⌕ Search

bioRxiv · 10.64898/2026.05.26.727992

Passive Acoustic Monitoring within the Northwest Forest Plan Area: 2025 Annual Report

Abstract

The Northwest Forest Plan (NWFP) Passive Acoustic Monitoring (PAM) program is a large-scale interagency biodiversity monitoring framework designed to assess the status and trends of northern spotted owls (Strix occidentalis caurina), barred owls (Strix varia), marbled murrelets (Brachyramphus marmoratus), and broader forest biodiversity across federally administered lands in the Pacific Northwest. In 2025, we deployed autonomous recording units at 2,095 sampling stations within 532 5-km2 hexagons randomly selected across approximately 24 million acres of federal forest lands in Washington, Oregon, and California. These deployments generated 1.37 million hours of acoustic recordings that we processed using the convolutional neural network PNW-Cnet v5 for automated species identification and subsequent human validation of focal species detections. Northern spotted owls were detected in 28% of sampled hexagons range-wide, including 16% in Washington, 25% in Oregon, and 60% in California. Occupancy patterns remained consistent compared to previous years, with higher occupancy concentrated in southern portions of the geographic range and continued low occupancy across much of the Washington Cascades and Oregon Coast Range. Barred owls were detected in 86% of sampled hexagons and remained broadly distributed throughout most of the NWFP area. Marbled murrelets were detected in 50% of reviewed hexagons within NWFP marbled murrelet management zones, with highest occupancy occurring in coastal forests of Oregon and Washington. The 2025 field season occurred under substantial operational constraints that reduced sampling effort by approximately half relative to 2023 and 2024 because of staffing limitations affecting participating federal agencies. Despite these reductions, the NWFP-PAM framework continued to provide broad-scale, spatially representative ecological information across the NWFP area. Results highlight the growing importance of passive acoustic monitoring and machine learning approaches for long-term biodiversity monitoring under changing environmental and operational conditions.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Lesmeister, D. B., Jenkins, J., Ruff, Z., Rugg, N., Abidari, S., Beavers, S., Christophersen, R., Davis, R. J., Terwilliger, M., Henderson, A., Henson, B., Kasper, J., McCafferty, C., Press, D., Reffler, S., Swingle, J., Thomas, A., Wert, K.. 2026-05-29. Passive Acoustic Monitoring within the Northwest Forest Plan Area: 2025 Annual Report. https://doi.org/10.64898/2026.05.26.727992

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

A leaf-carbon economy model to explain the adaptive value of low vs. high leaf mass per area as a function of habitat productivity

Plant traits interact with environmental conditions to determine plant fitness. In particular, plant species with low leaf mass per area (LMA) are more common in high fertility soils and forest gaps, while plant species with high LMA are more common in low fertility soils and in shaded forest understories. It is not yet known why the adaptive value of LMA changes as a function of environmental productivity. We present a model for plant growth as a function of LMA and productivity. Our model relies on an empirically derived relationship between LMA and leaf lifespan. We are able to explain why species with lower LMA are successful in high productivity environments including environments with high availability of either light or soil nutrients, by showing that the LMA which maximizes growth rate decreases with increasing rate of carbon assimilation. We show that low LMA species are better at taking advantage of the 'high interest' environment of high productivity habitats by reinvesting assimilated carbon in new leaves and earning compound interest, while high LMA species grow more efficiently in the 'low interest' environment of low productivity habitats. This tradeoff sheds light on the adaptive value of LMA as it relates to environmental productivity.

ecology↗

Species differentiate water uptake depths within a narrow vertical zone in temperate grasslands

Whether and under which conditions coexisting species in temperate grasslands differentiate their water uptake within the shallow soil layer they share is central to understanding hydrological niche differentiation in these ecosystems. In this field study, we quantified water uptake depth of 28 coexisting herbaceous species across 74 temperate grassland plots in three regions of Germany using oxygen stable isotopes and soil water content measured at multiple depths. Species consistently differed in water uptake depth across all regions, consistent with hydrological niche differentiation. This differentiation was largely confined to the upper 30 cm of soil and became stronger as overall soil moisture declined, with plants accessing water at greater depths in drier plots. In contrast, the vertical distribution of soil moisture was unrelated to either uptake depth or species differentiation. Despite pronounced species level variation, broad herbaceous functional classifications, including grasses versus forbs and fibrous versus taprooted species, showed substantial overlap in water uptake depth. Together, our results show that hydrological niche differentiation in temperate grasslands is expressed through fine scale differences among species within a shared shallow soil domain, becomes more pronounced under drier conditions, and is poorly captured by broad herbaceous functional classifications.

ecology↗

Fungal-bacterial interactions enhance bacterial but constrain fungal resistance and recovery to extreme heat across soil moisture levels

Soil fungi and bacteria are key drivers of nutrient cycling and plant productivity in terrestrial ecosystems, yet their responses to climate extremes are rarely studied as interacting partners rather than in isolation. Moreover, it remains largely unknown how fungal-bacterial interactions regulate fungal and bacterial stability under climate extremes, particularly resistance (immediate responses to climate extremes) and recovery (responses after climate extremes cease). Here, we conducted a soil microcosm experiment with a bacterial species (Pseudomonas putida KT2440) paired with one of four Trichoderma species (T. hamatum, T. atroviride, T. koningiopsis, and T. harzianum). These fungal-bacterial pairs were then exposed to a 5-day heat event (40 {degrees}C/32 {degrees}C, day/night) followed by a 14-day recovery period (25 {degrees}C/17 {degrees}C, day/night), at two soil moisture levels (30% and 5% gravimetric water content). Our results show that fungal-bacterial interactions asymmetrically affect fungal and bacterial responses to extreme heat. Trichoderma species facilitated the resistance and recovery of P. putida density (CFUs) to extreme heat, whereas P. putida constrained the resistance and recovery of fungal biomass (ergosterol) to extreme heat, regardless of soil moisture conditions. Moreover, faster-growing Trichoderma species were associated with greater facilitation of bacterial resistance and recovery. Bacterial density also covaried positively with fungal biomass during heat stress under both soil moisture conditions, suggesting that net fungal facilitation enhances bacterial persistence under extreme heat. Together, our findings reveal that fungal-bacterial interactions can stabilize one microbial partner while increasing the vulnerability of the other, highlighting the contrasting consequences of biotic interactions under climate extremes.

ecology↗