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bioRxiv · 10.64898/2026.05.21.726923

Generation and validation of an Acan-Cre mouse line to selectively label Class-B excitatory neurons of the cerebellar nuclei

Cheron, J.·Lowman, M.·Anant, M.·Siauw, M.·Kebschull, J. M.
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Abstract

The cerebellar nuclei form the main output structures of the cerebellum and are composed of a deeply conserved set of cell types. Two excitatory cell classes, Class-A and -B, are present in each cerebellar nucleus and mediate all excitatory output of the cerebellum. To provide genetic access to these cell types, here we identified Acan as a marker gene for Class-B cells and generated a knock-in Acan-P2A-Cre mouse line. We demonstrate that this Acan-Cre line selectively labels Class-B neurons in the cerebellar nuclei and validate its use in viral projection tracing. This new mouse line provides a valuable genetic tool to study cerebellar nuclei organization and function.

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Cheron, J., Lowman, M., Anant, M., Siauw, M., Kebschull, J. M.. 2026-05-23. Generation and validation of an Acan-Cre mouse line to selectively label Class-B excitatory neurons of the cerebellar nuclei. https://doi.org/10.64898/2026.05.21.726923

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