Dynamic visualization of physiological CaMKII activity using sensitive FRET biosensors

Mehta, S.; Thaker, N. A.; Adachi, K.; Ko, C. Y.; Liu, B.; Divkaruni, S. S.; Koc, O. C.; Lyons, A. C.; Sanchez, S. A.; Löffler, P.; Nakahata, Y.; Martin, J. L.; Johnson, J. L.; Yaron-Barir, T. M.; Cantley, L. C.; Lohse, M. J.; Bock, A.; Bers, D. M.; Yasuda, R.; Fissore, R.; Huganir, R. L.; Stratton, M. M.; Zhang, J.

doi:10.64898/2026.05.20.726522

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bioRxiv · 10.64898/2026.05.20.726522

Dynamic visualization of physiological CaMKII activity using sensitive FRET biosensors

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Abstract

Calcium-calmodulin (CaM)-dependent protein kinase II (CaMKII) is a key mediator of complex physiological processes throughout the body, from the brain to the reproductive system, where CaMKII translates spatiotemporally dynamic calcium elevations into specific biological functions. Directly visualizing CaMKII activity dynamics in living cells using genetically encoded fluorescent biosensors can thus provide crucial insights into the molecular regulation of health and disease. Yet the ability to sensitively and specifically monitor endogenous CaMKII activity in physiologically relevant contexts is limited by the lack of sensors that can achieve robust, quantitative visualization of CaMKII responses. Here, we leveraged a recent serine/threonine kinome-wide substrate atlas to rationally engineer a powerful suite of Forster resonance energy transfer (FRET)-based CaMKII kinase activity reporters with high specificity, sensitivity, and signal-to-noise ratio. Using these biosensors, we were able to sensitively and robustly visualize endogenous CaMKII activity dynamics in both cultured cell lines and primary cells, including cardiomyocytes, oocytes, and neurons. We further utilized 2pFLIM imaging of organotypic hippocampal slices to quantitatively track LTP-induced CaMKII activity within single dendritic spines, highlighting a major advance in the study of physiological CaMKII signaling.

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Mehta, S., Thaker, N. A., Adachi, K., Ko, C. Y., Liu, B., Divkaruni, S. S., Koc, O. C., Lyons, A. C., Sanchez, S. A., Löffler, P., Nakahata, Y., Martin, J. L., Johnson, J. L., Yaron-Barir, T. M., Cantley, L. C., Lohse, M. J., Bock, A., Bers, D. M., Yasuda, R., Fissore, R., Huganir, R. L., Stratton, M. M., Zhang, J.. 2026-05-22. Dynamic visualization of physiological CaMKII activity using sensitive FRET biosensors. https://doi.org/10.64898/2026.05.20.726522

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