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bioRxiv · 10.64898/2026.05.18.725864

Isolation of compounds from Cyathea podophylla and their cytoprotective effects against 6-hydroxydopamine-induced toxicity in F11 neuronal cells

Vu, B. L.·Lam, H.·Nguyen, L. D. L.·Do, C. P.·Trang, V. T. H.
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Abstract

The chemical constituents and cytoprotective potential of Cyathea podophylla, a Vietnamese fern, remain poorly investigated. This study aimed to isolate its compounds and evaluate their in vitro cytoprotective activity against 6-hydroxydopamine (6-OHDA)-induced toxicity in F11 cells. Compounds were chromatographically isolated and structurally characterized using NMR and HR-ESI-MS. Seven compounds were identified: five phenolics (trans-cinnamic acid, (E)-4-(3,4-dihydroxyphenyl)but-3-en-2-one, p-coumaric acid, 3,4-dihydroxybenzoic acid, 4-O-acetyl-caffeic acid), 5-hydroxymethylfurfural, and butyl-{beta}-D-fructofuranoside. Six of these are newly reported for the Cyathea genus. In MTT assays, butyl-{beta}-D-fructofuranoside exhibited the strongest cytoprotective effect (69.6% cell protection at 10 {micro}M, p < 0.001), followed by (E)-4-(3,4-dihydroxyphenyl)but-3-en-2-one (39.2% at 10 {micro}M). The remaining compounds lacked significant activity. These findings expand the phytochemical profile of Cyathea podophylla and provide preliminary evidence of its cytoprotective properties against 6-OHDA-induced injury, warranting further mechanistic and in vivo validation.

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Vu, B. L., Lam, H., Nguyen, L. D. L., Do, C. P., Trang, V. T. H.. 2026-05-20. Isolation of compounds from Cyathea podophylla and their cytoprotective effects against 6-hydroxydopamine-induced toxicity in F11 neuronal cells. https://doi.org/10.64898/2026.05.18.725864

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