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bioRxiv · 10.64898/2026.05.17.725740

Structure of the human HIRA histone chaperone with a nucleosome suggests a stepwise nucleosome assembly mechanism

Tian, W.·Chen, S.·Yao, L.·Kasinath, V.·Luger, K.
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Abstract

The restoration of chromatin in the wake of a DNA or RNA polymerase is essential to maintain the integrity of eukaryotic genomes. Human HIRA is a 1.8-megadalton, three-subunit histone chaperone that mediates all replication-independent deposition of the histone variant H3.3 at active chromatin regions1-6. Disruption of HIRA perturbs active-chromatin organization and has wide-ranging consequences for development, cellular senescence, and genome integrity7-11. Despite its central biological role in reassembling nucleosomes post-transcription, the structure of native human HIRA and the mechanism by which it organizes histones and DNA during nucleosome assembly remain unknown. In particular, the function of the largest HIRA subunit CABIN1 is enigmatic. Here, we show that HIRA is not simply a passive histone hand-off factor but remains engaged across multiple stages of nucleosome assembly, including a close interaction with the nucleosome. Cryo-EM structures reveal that HIRA forms an extended arch-like structure that binds the nucleosome primarily through extensive CABIN1 contacts with histones, histone tails, nucleosomal DNA, and linker DNA, during the final stage of nucleosome assembly. Together, our results suggest a testable mechanism for HIRA-mediated nucleosome assembly and product release and provide the basis for elucidating the molecular details of this fundamental biological process.

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Tian, W., Chen, S., Yao, L., Kasinath, V., Luger, K.. 2026-05-18. Structure of the human HIRA histone chaperone with a nucleosome suggests a stepwise nucleosome assembly mechanism. https://doi.org/10.64898/2026.05.17.725740

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