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bioRxiv · 10.64898/2026.05.17.725056

Oxytocin regulates TN-GnRH3 circuit maturation and mate preference through C1q-dependent synaptic mechanisms

Hasegawa, M.·Oshita, M.·Naruse, K.·Hodne, K.·Weltzien, F.-A.·Higashijima, S.-i.·Amano, T.·Minami, M.·Takeuchi, H.·Nakagawa, S.·Yokoi, S.
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Abstract

Oxytocin is a key regulator of social behavior, yet how it shapes the neural circuits underlying these behaviors remains unclear. Here, we show that oxytocin signaling is required for the proper maturation of terminal nerve gonadotropin-releasing hormone 3 (TN-GnRH3) circuits that regulate female mate preference in medaka. Disruption of oxytocin signaling reduced expression of the complement component C1q, altered microglial properties, and increased TN-GnRH3 innervation in the optic tectum and dorsolateral telencephalon, suggesting impaired synaptic refinement. In addition, TN-GnRH3 neurons in mutants exhibited disrupted clustering and failed to increase firing frequency in response to visual stimulation, indicating functional deficits. Furthermore, c1qb mutants phenocopied the behavioral and neuronal abnormalities observed in oxytocin signaling-deficient mutants. Together, our findings suggest that oxytocin signaling links microglia-associated synaptic refinement to neural circuit maturation, thereby revealing a previously unrecognized role for oxytocin in shaping social behavior.

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Hasegawa, M., Oshita, M., Naruse, K., Hodne, K., Weltzien, F.-A., Higashijima, S.-i., Amano, T., Minami, M., Takeuchi, H., Nakagawa, S., Yokoi, S.. 2026-05-18. Oxytocin regulates TN-GnRH3 circuit maturation and mate preference through C1q-dependent synaptic mechanisms. https://doi.org/10.64898/2026.05.17.725056

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